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| 不同氮、磷浓度对布氏双尾藻休眠孢子形成的影响 |
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李 朗1, 高亚辉1,2, 刘广发1,2, 梁君荣1,2, 赵 龙1, 胡 肖1, 徐华林3, 章 军1, 陈长平1,2
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1.厦门大学生命科学学院;2.滨海湿地生态系统教育部重点实验室;3.广东内伶仃-福田国家级自然保护区管理局
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| 摘要: |
| 有些海洋硅藻在不良的环境下会形成休眠孢子, 这种过程与环境中的氮、磷浓度密切相关。本文研究了培养基中不同氮、磷浓度下布氏双尾藻(Ditylum brightwellii)休眠孢子的形成过程。结果表明:氮浓度在27 μmol/L 以下时即可形成休眠孢子, 且培养基中氮初始浓度越低, 休眠孢子出现得越早; 单因子变量为磷浓度时, 各组均在第3 天形成休眠孢子, 且培养基中磷浓度变化不大, 其中磷浓度较高(50 μmol/L)时, 培养基中休眠孢子的密度和形成率均明显高于磷浓度较低的培养基(20 μmol/L 和10 μmol/L)。低氮高磷(氮: 20 μmol/L, 磷: 50 μmol/L)组休眠孢子密度和形成率在四组中最高, 说明布氏双尾藻休眠孢子的形成受氮、磷两种因子的共同影响。 |
| 关键词: 布氏双尾藻(Ditylum brightwellii) 氮 休眠孢子 磷 |
| DOI：10.11759/hykx20150206003 |
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| 基金项目:国家自然科学基金(NO. 41276100); 中国科学院战略性先导科技专项(XDA11020103); 福建省自然科学基金(2010J01232) |
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| Effects of different concentrations of nitrogen and phosphorus on the resting spore formation of Ditylum brightwellii (West) Grunow |
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LI Lang,GAO Ya-hui,LIU Guang-fa,LIANG Jun-rong,ZHAO Long,HU Xiao,XU Hua-lin,ZHANG Jun,CHEN Chang-ping
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| Abstract: |
| In some species of diatoms, resting spore formation is a common phenomenon observed during adverse environmental conditions and is usually related to the level of nitrogen (N)and phosphorus (P). This study presents the effects of different concentrations of N and P on Ditylum brightwellii (West)Grunow resting spore formation. We counted the resting spores, calculated the resting spore densities, and quantified the nutrients in the medium. The results revealed that D. brightwellii formed resting spores when the N concentration in the culture medium fell below 27 μmol/L. Moreover, D. brightwellii formed resting spores earlier when the initial N concentration was low. Resting spores all appeared at day 3 when P concentration was the single variable factor. P concentrations in these groups changed slightly. Under higher P concentrations (50 μmol/L), the density and ratio of resting spores were both markedly higher than those under lower P concentrations (20 μmol/L and 10 μmol/L). The group with the lowest N and highest P concentrations (N: 20 μmol/L, P: 50 μmol/L)had the highest density and ratio of spores; thus, N and P had a mutual influence on resting spore formation. |
| Key words: Ditylum brightwellii Nitrogen Resting spores Phosphate |