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| Magnetospira sp. QH-2 MamK蛋白的结构和系统进化分析 |
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滕兆洁1,2,3, 张文燕1,3, 刘 佳1,2,3, 董 逸1,3, 陈一然1,3, 潘红苗1,3, 杜海舰1,3, 王明玲1,3, 徐 丛1,2,3, 肖 天1,3
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1.中国科学院 海洋研究所, 海洋生态与环境科学重点实验室;2.中国科学院大学;3.青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室
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| 摘要: |
| Magnetospira sp. QH-2是一株分离自黄海潮间带的海洋趋磁螺菌, 该菌磁小体排列不整齐, 并且磁小体链内某些区域排列疏松, 推测与其磁小体岛上的mamK编码的MamK蛋白相关。mamK开放阅读框大小为1086 bp, 编码361个氨基酸, MamK蛋白属于NBD_sugar-kinase_HSP70_actin超家族, 为非跨膜蛋白; 此外, 还利用生物信息学工具预测MamK蛋白二级和三级结构。系统进化分析显示MamK蛋白的系统进化和16S rRNA基因的系统进化有一定差异, 推测磁小体岛的获得可能与菌种的进化是独立的两个过程; 淡水与海水中的趋磁螺菌虽然形态相似, 但系统进化地位有一定差异, 可能是两者不同生境下适应性演化的结果。 |
| 关键词: MamK 系统进化 16S rRNA 基因 磁小体岛 结构 |
| DOI：10.11759/hykx20151103001 |
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| 基金项目:国家自然科学基金(41206150, 41276170, 41330962) |
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| Insight into the structure and evolution of the actin-like protein MamK in Magnetospira sp. QH-2 |
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TENG Zhao-jie,ZHANG Wen-yan,LIU Jia,DONG Yi,CHEN Yi-yan,PAN Hong-miao,DU Hai-jian,WANG Ming-ling,XU Cong,XIAO Tian
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| Abstract: |
| Magnetospira sp. QH-2 is a marine magnetotactic spirillum, isolated from the intertidal zone of the Yellow Sea, China. The trait that its magnetosome chains display a relatively large size distribution is predicted to be related to the function of the mamK-encoded protein, namely, MamK. We find that MamK gene codes for 1086bp and MamK, which belong to the NBD_sugar-kinase_HSP70_actin superfamily, are composed of 361 amino acids and do not contain any trans-membrane domain. Based on the differences of the phylogenetic trees, we determine that the acquisition of magnetosome island and bacteria evolution are possibly two independent processes. While the morphology feature of magnetotactic spirillum both in fresh water and marine is similar, its phylogenetic status is different to some extent, which may indicate its adaptive evolutions. |
| Key words: MamK phylogenesis 16S rRNA gene Magnetosome Island structure |