| 摘要: |
| 羊栖菜(Sargassum fusiforme)是一种有“长寿菜”美誉的经济藻类, 其中的多糖成分具有良好的抗氧化活性。本文依次用冷水和热水处理羊栖菜藻粉, 得到两种多糖: 冷水浸提多糖(SFCP)和热水煮提多糖(SFHP); 随后对这两种多糖的理化性质进行比较, 并通过测定二者对DPPH 和羟自由基的清除能力评价其抗氧化活性。结果表明, 两种多糖都是含有硫酸根的岩藻聚糖, 但是SFCP 中糖醛酸含量较高,分子质量分布集中, 而SFHP 中硫酸根含量较多, 分子质量分布较宽, 且二者硫酸根取代位置不同。两种多糖对DPPH 和羟自由基均有一定的清除效果, 且清除率与浓度存在剂量依赖关系。相比较而言,SFCP 对自由基的清除效率较好。 |
| 关键词: 羊栖菜 多糖 抗氧化 |
| DOI：10.11759/hykx20161205 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目(31470430, 31200266); 温州市科技计划项目(N20150034) |
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| Extraction and antioxidative activity of fucoidan from Sargassum fusiforme |
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HE Dan,ZHANG Xu,XIAO Bao-heng,WU Xiao-hui,WU Ming-jiang
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| Abstract: |
| Sargassum fusiforme is an alga with economic significance and a good reputation for food longevity. Its polysaccharide also demonstrates good biological activities. In this study, we extracted two polysaccharide fractions—S. fusiforme cold-water-extracted polysaccharide (SFCP) and S. fusiforme hot-water-extracted polysaccharide (SFHP)—from S. fusiforme with water at 4℃ and 80℃, successively. We determined the physical and chemical properties of the polysaccharide fractions and evaluated their anti-oxidative activities by the radical scavenging ratios of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free and hydroxyl radicals. The results show that the two polysaccharide fractions were both fucosan with sulfate. SFHP showed a wider molecular weight distribution than SFCP and contained more sulfate, whereas SFCP contained more uronic acid. Their sulfate radical substitution positions also differed. SFHP and SFCP demonstrated certain dose-dependent activities for scavenging DPPH and ·OH. SFCP demonstrated better efficiency in removing free radicals. |
| Key words: Sargassum fusiforme polysaccharide antioxidant |
