| 摘要: |
| 为了解许氏平鲉(Sebastes schlegelii)微卫星标记与生长性状的相关性, 选取26个微卫星标记在海捕许氏平鲉群体中进行微卫星筛选, 每个微卫星位点的等位基因数(Na)在3～21, 有效等位基因数(Ne)为1.1815～15.5676; 观测杂合度(Ho)范围为0.0417～0.9999; 期望杂合度(He)范围为0.1581～0.9456;多态信息含量(PIC)范围为0.1509～0.9320。利用单标记分析法分析了多态性较高(PIC>0.5)的22个标记与体长、体质量、体高的相关性, 结果显示3个标记与生长性状显著相关, 其中HJ2-32与体长、体质量和体高显著相关(P<0.05), 并推断等位基因D与C对于体质量、体长、体高分别具有重要的正向与负向影响; HJ7-2与体质量显著相关; HJ7-22与体长显著相关, 并推测等位基因F对体长具有正向影响,等位基因B对体长有负面影响。这些标记可为许氏平鲉分子标记辅助育种提供基础。 |
| 关键词: 许氏平鲉(Sebastes schlegelii) 微卫星标记 生长性状 相关性分析 |
| DOI：10.11759/hykx20161209 |
| 分类号: |
| 基金项目:山东省良种工程项目(2014-2017) |
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| Correlation analysis of microsatellite markers with body length, weight, and height in Sebastes schlegelii |
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HAN Cheng-hui,MA Hai-tao,XUE Rui,JIANG Hai-bin,CHANG Cheng,WANG Teng
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| Abstract: |
| Twenty-six polymorphic microsatellite markers were selected to estimate a wild population of Sebastes schlegelii for studying the correlation between microsatellite markers and growth traits, which revealed that the number of alleles was 260 and the allele numbers ranged from 3 to 21 per locus. The observed and expected heterozygosity varied from 0.0417 to 0.9999 and from 0.1581 to 0.9456, respectively. The values of polymorphic information content ranged from 0.1509 to 0.9320. Twenty-two microsatellite loci with high polymorphism were used to analyze the correlation with body length, weight, and height by single marker-based analysis. The results showed that these markers significantly correlated with growth traits; loci HJ2-32 significantly correlated with three growth traits, and alleles D and C had positive and negative effects on growth traits, respectively. HJ7-2 showed a significant correlation with body weight; alleles F and B of HJ7-22 had positive and negative effects on length traits, respectively. These aforementioned markers would be of benefit for a further study on molecular marker-assisted breeding of S. schlegelii. |
| Key words: Sebastes schlegelii microsatellite growth traits correlation analysis |
