| 摘要: |
| 为了筛选纯化紫菜类菌孢素氨基酸(mycosporine-like amino acid, MAAs)的离子交换树脂, 优化纯化工艺, 并研究纯化后MAAs体外抗紫外辐射活性, 本研究通过静态动力学吸附及解析实验, 筛选纯化树脂; 结合动态动力学吸附及解析单因素实验建立响应面法对SA-2型阳离子树脂纯化MAAs的二次多项式模型, 对MAAs的纯化工艺进行优化; 通过建立大肠杆菌、金黄色葡萄球菌的复合紫外线(UVA+UVB)损伤模型, 分析纯化后MAAs样品的体外抗辐射活性。结果表明: 静态动力学吸附及解析实验筛选出SA-2型阳离子树脂为纯化MAAs的最佳树脂, 吸附率和解析率分别为86.01%和56.63%;吸附的最佳工艺条件为: 上样液质量浓度3 g/L、上样流速1 mL/min、上样液pH为6、洗脱液体积分数为3%、洗脱流速为1.5 mL/min、洗脱液pH为6; 在此条件下吸附率为79.21%, 洗脱率为64.02%; 纯化后的MAAs样品能有效抑制紫外辐射而造成的损伤, 将菌种失活速度分别降低了39.10%和40.58%,表现出显著的抗紫外辐射活性。 |
| 关键词: 坛紫菜 类菌孢素氨基酸 SA-2 树脂 纯化 响应面 抗紫外辐射 |
| DOI：10.11759/hykx20160811002 |
| 分类号: |
| 基金项目:国家自然科学基金(31272705); 海洋公益性行业科研专项(201505022-5) |
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| Optimization of purification process of mycosporine-like amino acid from Porphyra haitanensis and study on its antiultraviolet activity |
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YING Rui,ZHANG Zhao-hui,DUAN Xiao-shan,ZHAO Xue,HOU Hu,LI Ba-fang
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| Abstract: |
| Objective: To explore the optimal techniques in the separation of mycosporine-like amino acids (MAAs) in Porphyra haitanensis with cation-exchange resins and the study on its anti-ultraviolet activity after purification. Method: In the screening cation-exchange resin through the static adsorption and desorption analytical experiment, response-surface methodology was employed to establish a mathematical regression model with the dynamic adsorption and desorption in the single-factor experiments for SA-2. The ultraviolet-radiation models (UVA and UVB) when compared with blank samples showed that the survival rates of the MAAs groups of E. coli and S. aureus increased; this proved that the MAAs after purification had certain anti-ultraviolet activity. Result: SA-2 was found to be the most suitable resin for the mycosporine-like amino acid purification; the obtained adsorption and desorption rates were 86% and 56.63%, respectively. The optimum conditions for the purification were obtained when the sample containing 3 g/L MAAs at pH 6 was mounted onto a column at a flow rate of 1 mL/min; further, it was eluted with 3% ammonium hydroxide to pH 6 at a flow rate of 1.0 mL/min. The purification process yielded an adsorption and desorption rate of 79.21% and 64.02%, respectively. After the purification of MAAs sample, it had significant resistance to the ultraviolet radiation and the deactivation rate for E. coli and S. aureus decreased to 39.10% and 40.58%, respectively. |
| Key words: Porphyra haitanensis mycosporine-like amino acids SA-2 resin purification response surface ultraviolet-resistant activity |
