| 摘要: |
| 以三疣梭子蟹肌肉基本营养成分、蛋白质组成、肌原纤维蛋白含量、Ca2+-ATPase活性、总巯基含量、二硫键含量以及肌原纤维蛋白的SDS-PAGE分析作为指标, 研究了三疣梭子蟹在不同冻藏温度下肌肉蛋白质生化特征的变化。结果表明, 三疣梭子蟹是典型的高蛋白食品; 随着冻藏时间的延长, 水溶性蛋白含量先增加后减少, 盐溶性蛋白和不溶性蛋白含量逐渐减少, 碱溶性蛋白含量逐渐增加; 肌原纤维蛋白含量、Ca2+-ATPase活性、总巯基含量随着冻藏时间的延长, 均呈现下降趋势, 而二硫键含量则呈上升趋势, 且–20℃和–40℃两组之间差异显著(P<0.05)。SDS-PAGE分析结果表明, 组成肌原纤维蛋白的各种蛋白质均有不同程度降解, 且–20℃比–40℃组降解更明显。因此, –40℃冻藏对梭子蟹肌肉蛋白质生化特性的影响较小。 |
| 关键词: 三疣梭子蟹(Portunus trituberculatus) 肌肉 蛋白质 生化特性 |
| DOI：10.11759/hykx20151224003 |
| 分类号: |
| 基金项目:山东省自然科学基金项目(ZR2015CM010) |
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| Changes in the biochemical properties of muscle protein of swimming crab (Portunus trituberculatus) during frozen storage |
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HUANG Lin,ZHANG Li,WU Dan-lu
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| Abstract: |
| This study analyzed the changes in the biochemical characteristics of muscle protein during frozen storage at –20℃ or –40℃ based on nutrient composition, protein composition, myofibrillar protein content, Ca2+-ATPase activity, sulfhydryl group content, disulfide bond content, and SDS-PAGE analysis of myofibrillar protein of swimming crab. Results showed that the muscle of the swimming crab was a typical food with a high protein content. With extension of the duration of frozen storage, the water-soluble protein content increased and then decreased, the salt-soluble protein and -insoluble protein contents decreased, and the content of alkaline-soluble protein increased gradually. Myofibrillar protein content, Ca2+-ATPase activity, and sulfhydryl group content decreased with prolongation of the duration of frozen storage, whereas the disulfide bond content showed an increasing trend, with a significant difference between the two groups at –20℃ and –40℃ (P<0.05). The SDS-PAGE results showed that all types of proteins of myofibrillar protein were degraded to different degrees, and the degradation was more apparent at ?20℃ than at ?40℃. Therefore, frozen storage at –40℃ has minimal effect on the biochemical characteristics of muscle protein. |
| Key words: swimming crab (Portunus trituberculatus) muscle protein biochemical properties |
