| 摘要: |
| 为开发安全、高效的防治大菱鲆(Scophthalmus maximus)盾纤毛虫(scuticociliate)病新型药物, 本研究从近海微生物菌种资源库中筛选获得了一株有杀盾纤毛虫效果的细菌, 利用形态学和分子生物学方法鉴定该菌为肋生盐弧菌(Salinivibrio costicola), 通过共培养实验进行YCSC6杀灭大菱鲆病原性盾纤毛虫的药效活性实验, 同时利用光学显微镜和扫描电镜观察记录YCSC6的杀虫特征。结果发现海洋细菌YCSC6发酵上清液可导致盾纤毛虫膜出现穿孔, 膜完整性丧失从而裂解, 在一定时间内, 发酵液发酵时间越长, 裂解能力越强。本研究从安全、高效的角度出发, 筛得一株可裂解盾纤毛虫的海洋细菌YCSC6, 并初步探究了其杀虫特征及能力, 为养殖渔业微生物杀虫剂的开发提供了安全有效的来源。 |
| 关键词: 大菱鲆(Scophthalmus maximus) 盾纤毛虫(scuticociliate) YCSC6 裂解 微生物杀虫剂 |
| DOI：10.11759/hykx20161202001 |
| 分类号: |
| 基金项目:十三五海洋环境安全保障重点专项(2017YFC1404504); 山东省自主创新及成果转化专项(2014ZZCX06205); 青岛海洋科学与技术国家实验室鳌山科技创新计划(2015ASKJ02-03) |
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| Identification of marine bacterium YCSC6 and its insecticidal ability against Scuticociliate in vitro |
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DU Guang-xun,QU Ling-yun,SHANG Kun,WANG Chen,GAO Ping
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| Abstract: |
| To develop a safe and effective new drug for the prevention and control of turbot scuticociliate disease, from the library of offshore marine microbial resources we screened out a marine bacterium that showed insecticidal activity against scuticociliate in vitro. We identified YCSC6 as Scophthalmus maximus using morphological and molecular biological methods. We conducted co-culture pharmacodynamics experiments in which YCSC6 killed pathogenic scuticociliate. We characterized the insecticidal properties of YCSC6 by optical and scanning electron microscopy. The results show that the fermentation supernatant of YCSC6 can lead to membrane perforation in scuticociliate, after which the scuticociliate loses its membrane integrity and cracks. After a certain period of time, the cracking ability strengthens with time. In this research, we made preliminary explorations of the insecticidal characteristics and ability in vitro of YCSC6 and found it to be a safe and effective source for the development of a microbe insecticide for aquaculture. |
| Key words: turbot scuticociliatlda YCSC6 lytic process microbe insecticide |
