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| 养殖水体中氨氮对大菱鲆的急性毒性效应研究 |
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李之乡1,2,3, 王伟继2,3, 胡玉龙2,3, 吕 丁2,3, 吴欢欢1,2,3, 栾 生2,3, 孔 杰1,2,3
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1.上海海洋大学 水产与生命学院;2.中国水产科学研究院 黄海水产研究所 农业部海洋渔业可持续发展重点实验室;3.青岛海洋科学与技术国家实验室 海洋渔业科学与食物产出过程功能实验室
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| 摘要: |
| 为了评估大菱鲆(Scophthalmus maximus)耐氨氮性状的遗传参数和为循环水养殖大菱鲆提供科学指导, 作者研究了养殖海水中氨氮对4月龄和8月龄大菱鲆的急性毒性效应, 同时对比了氨氮对白化和正常个体、雌性和雄性个体的急性毒性效应差别。结果表明: 非离子氨对4月龄大菱鲆的24、48、72、96 h LC50分别为2.19、1.94、1.80、1.72 mg/L, 对8月龄大菱鲆的24、48、72、96 h LC50分别为3.64、3.02、2.93、2.86 mg/L, 非离子氨对4月龄和8月龄大菱鲆的安全质量浓度分别为0.17和0.29 mg/L;氨氮对白化和正常大菱鲆以及雌性和雄性大菱鲆的急性毒性效应均没有显著性差异, 研究结果为进一步选育大菱鲆的耐氨氮品系提供科学依据。 |
| 关键词: 大菱鲆(Scophthalmus maximus) 苗种 氨氮 急性毒性 半致死浓度 白化 |
| DOI：10.11759/hykx2017041003 |
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| 基金项目:泰山学者种业计划专家项目(ZR2014CQ001); 山东省重点研发计划项目(2016ZDJS06A07); 国家高技术研究发展计划(863)(2012AA10A408-7) |
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| Acute toxicity of ammonia nitrogen on turbot (Scophthalmus maximus) |
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LI Zhi-xiang,WANG Wei-ji,HU Yu-long,LÜ Ding,WU Huan-huan,LUAN Sheng,KONG Jie
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| Abstract: |
| In this paper, we investigated the acute toxic effects of ammonia nitrogen on 4-month-old and 8-monthold turbot (Scophthalmus maximus), and compared the acute toxicity effects of ammonia nitrogen on albino and normal female and male turbot. The results showed that the median lethal concentrations (LC50) at 24, 48, 72, and 96 h for un-ionized ammonia on 4-month-old turbot were 2.19, 1.94, 1.80, and 1.72 mg/L, respectively. The LC50 values at 24, 48, 72, and 96 h for un-ionized ammonia on 8-month-old turbot were 3.64, 3.02, 2.93, and 2.86 mg/L, respectively. The safe concentrations of non-ionic ammonia on 4-month-old and 8-month-old turbot were 0.17 mg/L and 0.29 mg/L, respectively. Although the 8-month-old turbot was more tolerant to ammonia nitrogen than the 4-monthold turbot, there was no significant difference in either the correlation between body weight and ammonia resistance or body length and ammonia resistance. A comparison of the ammonia nitrogen acute toxicity in albino and normal female and male turbot revealed no significant differences. The results obtained in this study provide scientific guidance for the circulation water farming of turbot and a theoretical basis for evaluating the genetic parameters of ammonia-resistant traits and further selection breeding of ammonia-nitrogen-resistant strains of turbot. |
| Key words: Scophthalmus maximus fry ammonia acute toxicity LC50 albino |