| 摘要: |
| 本文研究了氨氮对方斑东风螺(Babylonia areolata)的急性毒性及不同浓度的氨氮溶液(22、47.5、102和220 mg/L)对其体内六种免疫酶(CAT、ACP、AKP、T-SOD、GSH-PX和POD)活性的影响。实验结果表明：氨氮浓度越大,其毒性作用越强,方斑东风螺死亡率越高；500 mg/L氨氮处理组于24 h便出现(20.3±2.1)%死亡率,并且96 h后存活率降为0。而相同条件下,随试验时间的延长,方斑东风螺死亡率也越高。氨氮浓度和处理时间均对方斑东风螺免疫酶活力有显著的影响(P < 0.05)。与对照组相比,处理组CAT和AKP活性均表现出“诱导—抑制”的趋势,ACP和T-SOD活性表现出“抑制—诱导”的趋势,CSH-PX活性呈现“诱导—抑制—诱导”的趋势,而氨氮对处理个体POD活性的影响整体表现为抑制作用。可见,养殖水体中的氨氮会对方斑东风螺免疫酶活性产生较大的影响。本研究从生态现象和生理指标相结合的方式阐述了氨氮对方斑东风螺个体的致毒特性,将对方斑东风螺的养殖具有实用意义,充实了氨氮对贝类毒理实验免疫性能层面的基础数据,可为其他贝类氨氮胁迫实验提供数据基础和科学依据。 |
| 关键词: 方斑东风螺(Babylonia areolata) 氨氮胁迫 免疫酶 |
| DOI： |
| 分类号:S917.4 |
| 基金项目:现代农业产业技术体系建设专项资金(CARS-49)；广东省渔港建设和渔业产业发展专项（海洋渔业科技推广方向-科技攻关与研发项目A201601B11）；海南省自然科学基金青年基金项目（318QN304）；三亚市农业科技创新项目（2018YD19） |
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| Effects of ammonia nitrogen stress on the activities of 6 immune enzymes of Babylonia areolata |
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TAN Chun-ming1, ZHAO Wang1, Wu Kai-chang1, ZHANG Yue2, YANG Rui1, Wen Wei-geng1, Chen Xu1, YU Gang1
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1.South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;2.College of Marine Science,Hainan University
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| Abstract: |
| In this paper, the acute toxicity of ammonia nitrogen and the effects of stress of different ammonia nitrogen concentrations (0, 22, 47.5, 102 and 220 mg/L) on the activities of 6 immune enzymes (CAT, ACP, AKP, T-SOD, GSH-PX and POD) of Babylonia areolata were studied. The results show that the greater the concentration of ammonia nitrogen, the stronger the toxic effect and the higher the mortality of B. areolata. On the treatment of 500 mg/L ammonia nitrogen group, (20.3±2.1)% mortality occurred at 24 h, and the survival rate decreased to 0 after cultured for 96 h. At the same time, the mortality of B. areolata is higher with the extension of the test time. And the results of immunoenzyme activities show that both the concentrations of ammonia nitrogen and the treatment time were found to have a significant effect on the activity of immune enzymes of B. areolata (P < 0.05). Compared with the control groups, the CAT and AKP activities show a trend of "induction - suppression", and the ACP and T-SOD generally demonstrated a "suppression - induction" activity. However, with the extension of ammonia nitrogen treatment time, CSH-PX activity shows a trend of “induction - suppression - induction”, while the effect of ammonia nitrogen on the POD activity of treated individuals show an inhibitory effect. It can be seen that the ammonia nitrogen in the aquaculture water has a greater impact on the immunoenzymatic activity of B. areolata. In this study, the combination of ecological phenomena and physiological indicators was used to describe the toxicity characteristics of the ammonia nitrogen to individual B. areolata, it will have practical significance for the culture of B. areolata. And the results will enrich the basic data of ammonia nitrogen on the immunity of shellfish toxicology experiments, which can provide data basis and scientific basis for other shellfish of ammonia nitrogen stress experiments. |
| Key words: Babylonia areolata ammonia- nitrogen stress immune enzymes |