| 摘要: |
| 为了分析人工养殖军曹鱼（Rachycentron canadum）亲鱼营养储备情况与性腺发育的相关性，作者采用生化的方法，对其肌肉、肝脏及性腺氨基酸含量进行测定。结果表明：（1）雄性亲鱼从Ⅳ期发育到Ⅴ期的过程中，精巢和肝脏牛磺酸含量、精巢总氨基酸含量均呈显著递增（P<0.05）；肌肉氨基酸总量呈显著下降趋势（P<0.05）；而肝脏氨基酸总量则无显著性差异（P>0.05）；（2）雌性亲鱼从Ⅳ期发育到Ⅴ期的过程中，肝脏牛磺酸含量呈显著升高（P<0.05）；卵巢无显著变化（P>0.05）；总氨基酸含量差异变化规律与雄性亲鱼相类似。（3）亲鱼总氨基酸含量、必需氨基酸含量在不同组织间存在显著差异（P<0.05），表现为肌肉含量最高，其次是性腺，肝脏中最低。肌肉、肝脏及性腺氨基酸组成类似，含量较高的为谷氨酸、天冬氨酸和赖氨酸；含量较低的为组氨酸、蛋氨酸和半胱氨酸。（4）发育处于同期性腺雌雄亲鱼，雄性亲鱼肌肉、性腺必需氨基酸含量和总氨基酸含量显著高于雌性亲鱼（P<0.05），而肝脏必需氨基酸含量总和氨基酸含量无显著性变化（P>0.05）。 |
| 关键词: 军曹鱼(Rachycentron canadum) 肌肉 肝脏 性腺 氨基酸含量 |
| DOI：10.11759/hykx20181022003 |
| 分类号:S965.3 |
| 基金项目:现代农业产业技术体系专项资金资助（CARS-47）；广东海洋大学大学生创新创业训练计划项目（CXXL2018131）；广东海洋大学研究生基地项目（粤教研函[2016]39号） |
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| Analysis of amino acid composition of different tissues collected from cultured cobia (Rachycentron canadum) broodstock |
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HUANG Jian-sheng1, LU Zhi1, ZHANG Jian-dong1, CHEN Gang1, PAN Chuan-hao1, DENG Wen-xin2
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1.Fishery College, Guangdong Ocean University, Zhanjiang 524025, China;2.Guangdong Yuehai Feed Group, Zhanjiang 524000, China
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| Abstract: |
| In this study, amino acid composition of the muscle, liver, and ovary of mature fish (Rachycentron canadum, maturation stages Ⅳ and Ⅴ) was determined using biochemical methods to analyze the correlation between nutrient reserves of broodstock and gonadal development. It was found that (1) taurine levels in the testis and liver and total amino acid content in the testis had significantly increased (P<0.05) between stages Ⅳ and Ⅴ in male broodstock; the total amino acid content in the fish muscle had significantly decreased (P<0.05), whereas there was no significant difference in terms of the total amino acid content in the liver (P>0.05). (2) Taurine levels significantly increased in the liver (P<0.05) but not in the ovary (P>0.05) in females during gonadal development from stages Ⅳ to Ⅴ. The variation in the total amino acid content in females was similar to that in males. (3) The total amino acid and total essential amino acid content showed significant differences (P<0.05) among different tissues, with the highest level being detected in the muscle and the lowest in the liver. The amino acid content in the muscle, liver, and gonads were similar, with high levels of glutamic acid, aspartic acid, and lysine and low levels of histidine, methionine, and cysteine. (4) Male broodstock showed significantly higher total essential amino acid and total amino acid content in the muscle and gonad than female broodstock (P<0.05), whereas no significant difference was found in terms of this in the liver (P>0.05). |
| Key words: Rachycentron canadum muscle liver ovary amino acid composition |
