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| 番红砗磲外套膜色素提取条件优化及成分分析 |
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付正祎1, 刘二田1, 顾志峰1, 石耀华1, 王爱民1,2, 吴川良3, 刘春胜1
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1.海南大学海洋学院, 海南 海口 570228;2.海南大学南海海洋资源利用国家重点实验室, 海南 海口 570228;3.三亚珊瑚礁生态研究所, 海南 三亚 572000
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| 摘要: |
| 以番红砗磲（Tridacna crocea）为实验对象，通过单因素对比实验对其外套膜色素的提取条件进行了优化，并利用薄层层析色谱法对色素组成进行了初步分析。结果表明：丙酮和乙醇为适宜的番红砗磲外套膜色素提取剂；使用丙酮为提取剂时，浸提时间为4 h，提取温度为25℃，料液比为1：40。使用乙醇为提取剂时，浸提时间为4 h，提取温度为30℃，料液比为1：200；利用薄层色谱法分离外套膜颜色为蓝色和红棕色的番红砗磲外套膜色素后发现，蓝色番红砗磲外套膜色素由9种色素组分构成，红棕色个体番红砗磲外套膜色素由10种色素组分构成。 |
| 关键词: 番红砗磲(Tridacna crocea) 外套膜色素 提取条件 薄层层析色谱 |
| DOI：10.11759/hykx20190207001 |
| 分类号:S917.4 |
| 基金项目:海南省重点研发计划项目（ZDYF2019153）；国家重点研发计划项目（2018YFD0900704）；海南省自然科学基金项目（317029） |
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| Optimization of extraction conditions of pigments in Tridacna crocea mantle and its composition analysis |
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FU Zheng-yi1, LIU Er-tian1, GU Zhi-feng1, SHI Yao-hua1, WANG Ai-min1,2, WU Chuan-liang3, LIU Chun-sheng1
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1.Ocean College, Hainan University, Haikou 570228, China;2.State Key Laboratory of Marine Resource Utlization in South China Sea, Hainan University, Haikou 570228, China;3.Ecologic Sanya Institute of Coral Reef Ecosystem, Sanya 572000, China
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| Abstract: |
| The extraction conditions of total pigments in Tridacna crocea mantle were optimized by single factor experiment, then the pigments composition were also preliminarily analyzed by thin layer chromatography. Results showed that acetone and ethanol were suitable solvents for the extraction of mantle pigments. When acetone was selected as the extractant, the extraction time, temperature and solid-liquid ratio were 4h, 25℃ and 1:40, respectively. When ethanol was selected as the extractant, the extraction time, temperature and solid-liquid ratio were 4h, 30℃ and 1:200, respectively. Thin Layer Chromatography was used to separate and compare the pigment components of reddish brown and blue individuals. It was found that the mantle pigments in reddish brown mantle were composed of 10 kinds of pigment components, while the pigments of blue mantle were composed of 9 kinds of pigment components. |
| Key words: Tridacna crocea pigment of mantle extraction condition thin layer chromatography |