| 摘要: |
| 以织锦巴非蛤（Paphia textile）为研究对象，分别用湿法与干法（冷冻干燥法）两种前处理方法提取织锦巴非蛤斧足、外套膜、闭壳肌及鳃4种组织的总类胡萝卜素，并比较两种前处理方法的提取效果。结果表明：用两种前处理方法提取各组织中总类胡萝卜素的变异系数均较小，干法的变异系数普遍小于湿法（P<0.05）。湿法处理后提取总类胡萝卜素含量从高到低依次为：斧足、鳃、外套膜、闭壳肌，干法处理后提取总类胡萝卜素的排序结果与湿法处理后提取的结果一致，但干法的得率要显著高于湿法（P<0.05），建议以后在对贝类中总类胡萝卜素进行提取时，宜选用冷冻干燥法作为前处理方法。 |
| 关键词: 织锦巴非蛤(Paphia textile) 总类胡萝卜素 冷冻干燥法 湿法 |
| DOI：10.11759/hykx20190430001 |
| 分类号:S917.4 |
| 基金项目:国家重点研发计划专项（2018YFD0901400） |
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| Comparison between two pretreatment methods of total carotenoid extraction from Paphia textile |
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ZHAO Hong-bo, DENG Su-zhen, ZHANG Jing, CAI Ming-yi, LIU Xian-de
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Fisheries College, Jimei University, Key Laboratory of Mariculture for the East China Sea, the Ministry of Agriculture and Rural Affairs of China, Xiamen 361021, China
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| Abstract: |
| Total carotenoid content (TCC) was extracted from four tissues of Paphia textile, including foot, mantle, adductor muscle, and gill, using the wet method and dry method (freeze-drying method) respectively, and the extraction effects of the two pre-treatment methods were compared. Results revealed smaller variation coefficients for the two pre-treatment methods, with the variation coefficient of the freeze-drying method being significantly smaller than that of the wet method (P<0.05). TCC extracted using the wet method ranged from high to low as the following order:foot, gill, mantle, and adductor muscle. The order of TCC extracted results using the freeze-drying method were same as wet method, but the yield of the freeze-drying method was significantly higher than that of wet method (P<0.05). It was suggested that the freeze-drying method should be used as a preferred pre-treatment method in the extraction of total carotenoids from shellfish in the future. |
| Key words: Paphia textile total carotenoids freeze-drying method wet method |
