| 摘要: |
| 安康鱼是一种低脂肪高蛋白的优质海洋生物资源，其氨基酸含量丰富，蛋白质含量高达75.30 %。本实验选用胰蛋白酶，胃蛋白酶，木瓜蛋白酶和菠萝蛋白酶水解安康鱼鱼糜，比较各酶解产物的DPPH自由基清除率，确定木瓜蛋白酶为最佳用酶。在单因素实验基础上进行响应面优化实验，确定酶解反应的最佳条件为：加酶量829.51U/g，pH值7.37，酶解温度47.09℃，酶解时间5.86h，液固比5.79：1。在最优条件下验证其DPPH自由基清除率为（80.22±0.63）%，接近预测值80.55%。经响应面法实验制备的安康鱼活性肽粉末呈淡黄色，具有较好的抗氧化活性，其羟自由基清除能力的IC50值为1.69 mg/mL，可作为潜在的抗氧化剂。 |
| 关键词: 安康鱼 酶解反应 木瓜蛋白酶 DPPH自由基清除率 响应面分析 |
| DOI： |
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| 基金项目:2020年度新旧动能转换重大课题攻关项目 |
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| Optimization of Monkfish Active Peptide Using Response Surface Methodology |
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WANG Xue-qin, TENG Li-chao, YU Hua-hua, XING Rong-e, LI Peng-cheng
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Institute of Oceanology, Chinese Academy of Sciences
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| Abstract: |
| Monkfish minced fillet is a kind of high-quality marine biological resource with high protein content and low fat content. It contains a variety of amino acids, and the protein content is up to 75.30 %. In this experiment, four kinds of proteases, i.e. trypsin, pepsin, papain and bromelain were used for hydrolysis to produce the monkfish protein hydrolysate, considering the DPPH radical scavenging activity of various enzymatic hydrolysates, the papain was choosen as the best enzyme. On the basis of single factor experiment, the optimal conditions were performed by the response surface methodology: enzyme concentration of 829.51 U/g, pH of 7.37, hydrolysis temperature of 47.09℃, hydrolysis time of 5.586 h and liquid-to-solid ratio of 5.79:1. The maximum DPPH scavenging activity was (80.22±0.63) %, which was similar to the experimental value of 80.55 %. The monkfish peptide prepared by response surface experiment is a light yellow powder with good antioxidant activity. Its IC50 of hydroxyl radical scavenging ability is 1.69 mg/mL, which can be used as a potential antioxidant. |
| Key words: Monkfish enzymatic hydrolysis papain DPPH radical scavenging activity response surface methodology |