| 摘要: |
| 为了研究三门湾海域温排水污染对水生动物发育的影响,本实验通过室内模拟三门湾核电站温排水的余氯残留和温升效应,研究了不同温度和不同游离余氯质量浓度耦合下对泥蚶(Tegillarca granosa)胚胎发育的影响。实验水温为29℃、32℃和35℃,游离余氯质量浓度为0.025、0.050、0.100、0.200、0.400和0.800 mg/L,同时以砂滤海水为对照组,选取30 min胚胎细胞组成、1 h卵裂率、24 h D型幼虫孵化率和幼虫畸形率等4个指标进行观察分析。结果表明,单一温升情况下,32℃对泥蚶30 min时细胞期发育有促进作用;35℃抑制了泥蚶30 min时的细胞期发育,泥蚶1 h卵裂率显著降低、无D型幼虫孵化。温度联合游离余氯情况下,3个温度下随着游离余氯质量浓度的增加,泥蚶受精卵卵裂率均呈下降趋势,游离余氯对泥蚶卵裂率影响的最低可观察效应浓度(LOEC)分别为0.400、0.200和0.800 mg/L。29℃和32℃下,随着余氯质量浓度的升高,D型幼虫孵化率呈下降趋势,畸形率呈升高趋势;两个温度下游离余氯对泥蚶D型幼虫孵化率和畸形率的最低可观察效应浓度(LOEC)分别为0.100和0.050 mg/L。35℃下,D型幼虫未孵出,无最低可观察效应浓度(LOEC)。本研究不仅可以为三门湾核电站海水中余氯污染对该海域生物和生态环境的影响提供基础资料,还可以为沿海电厂温排水排放标准提供理论指导意义。 |
| 关键词: 泥蚶(Tegillarca granosa) 胚胎发育 水温 游离余氯 |
| DOI：10.11759/hykx20210807001 |
| 分类号:X503.225 |
| 基金项目:国家重点研发计划项目(2020YFD0900802,2018YFD0901405-2);国家现代农业产业技术体系项目(CARS-49) |
|
| Toxic effects of water temperature and residual chlorine on embryonic development of Tegillarca granosa |
|
CHEN Lin1,2, TENG Shuang-shuang2, ZHU Ai-yi2, HUANG Xiao-lin2, XIAO Guo-qiang1,2
|
|
1.Zhejiang Ocean University, National Engineering Research Center For Marine Aquaculture, Zhoushan 316022, China;2.Zhejiang Mariculture Research Institute Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bioresource Wenzhou Key Laboratory of Marine Biological Genetics and Breeding, Wenzhou 325005, China
|
| Abstract: |
| To investigate the effects of warm drainage pollution in Sanmen Bay on the development of aquatic animals,the influence of free residual chlorine on the embryonic development of Tegillarca granosa under different temperature conditions was studied through laboratory simulations.Three different water temperatures were set at 29℃,32℃,nd 35℃,and six different gradients of free residual chlorine concentration were set at 0.025,0.050,0.100,0.200,0.400,and 0.800 mg/L.Simultaneously,filtered seawater was taken as the control.The influence of thermal drainage on the embryonic development of T.granosa was analyzed by counting the change of the embryonic cell composition,cleavage rate,D-shaped larva hatching rate,and malformation rate.The results of a single temperature rise revealed that 32℃ promoted cell development for 30 minutes after fertilization,whereas 35℃ inhibited cell development for 30 minutes after fertilization.The 1 h cleavage rate was significantly decreased,and the D-shaped larva hatching rate was zero at 35℃.Under the condition of temperature combined with free residual chlorine,the cleavage rate decreased along with the elevated concentration of free residual chlorine,and the lowest observed effect concentration (LOEC) values of free residual chlorine to the cleavage rate of T.granosa were determined to be 0.400,0.200,and 0.800 mg/L at 29℃,32℃,and 35℃,respectively.The D-shaped larva hatching rate decreased,and the malformation rate increased along with the elevated concentration of free residual chlorine at 29℃ and 32℃,respectively.The LOEC values of free residual chlorine to the hatching rate of T.granosa D-type larvae and the malformation rate were determined to be 0.100 mg/L and 0.050 mg/L,respectively.The D-shaped larva hatching rate was zero,and there was no LOEC value at 35℃.This study provides not only basic data for the impact of residual chlorine pollution on the biological and ecological environment in the Sanmen Bay Nuclear Power Plant but also theoretical guidance for formulating the discharge standard of warm water drainage of coastal power plants. |
| Key words: Tegillarca granosa embryonic development water temperature free residual chlorine |
