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| 一株分离自太平洋卡罗琳深海海山水域NX-5T菌株的多相分类鉴定 |
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刘杰1, 薛文萍1, 王玉璟1, 袁嘉琳1, 王龙1, 张德超2,3,4
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1.青岛科技大学海洋科学与生物工程学院生物工程系, 山东 青岛 266042;2.中国科学院海洋研究所, 山东 青岛 266071;3.中国科学院大学, 北京 100049;4.中国科学院海洋大科学中心, 山东 青岛 266071
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| 摘要: |
| 黄杆菌种类多、分布广,但从深海海山环境中发现黄杆菌新物种的报道还没有。2017年我们从西太平洋卡罗琳深海海山水域分离到一株细菌XN-5T,经16S rRNA基因序列测定表明,该菌与黄杆菌属(Flavobacterium)内系统发育关系最近模式菌株F.degerlachei LMG 21915T、F.frigoris LMG 21922T之间的相似性分别为97.5%和97.8%。本研究采用形态学观察、生理生化特征、遗传特征、脂肪酸和极性脂测定等方法,对该菌株进行了详细的多相分类鉴定。结果表明,XN-5T菌株在16S rRNA基因系统发育、生长温度、淀粉、酪蛋白水解、硝酸盐还原,产α-半乳糖苷酶、α-葡萄糖苷酶、β-葡萄糖苷酶,同化L-阿拉伯糖、D-甘露醇、N-乙酰-氨基葡萄糖、葡萄糖酸钾,发酵蔗糖、阿拉伯糖,以及脂肪酸、极性脂组成等方面,均与相近模式菌株有所差异。综合多相分类鉴定数据,我们判定XN-5T菌株为Flavobacterium属内的一个新种,命名为Flavobacterium pacifica sp. nov。 |
| 关键词: 太平洋卡罗琳海山 黄杆菌属 多相分类 |
| DOI：10.11759/hykx20201229002 |
| 分类号:Q346 |
| 基金项目:国家科技部基础性资源调查项目(2017FY100804) |
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| Polyphasic taxonomy of strain NX-5T isolated from the Caroline Seamount waters in the Pacific Ocean |
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LIU Jie1, XUE Wen-ping1, WANG Yu-jing1, YUAN Jia-lin1, WANG Long1, ZHANG De-chao2,3,4
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1.Department of Bioengineering, College of Marine Sciences and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;2.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;3.University of Chinese Academy of Sciences, Beijing 100049, China;4.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
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| Abstract: |
| Flavobacterium spp. are abundant with a wide distribution. However, new species of Flavobacterium have not been reported from the deep-sea seamount environment. In 2017, we isolated bacterial strain XN-5T from the Caroline Seamount waters of the Western Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain XN-5Thad the highest 16S rRNA gene sequence similarities with the type strains of F. frigoris LMG 21922T (97.8%) and F. degerlachei LMG 21915T (97.5%). In this study, we carried out polyphasic taxonomy of strain XN-5T, including morphological observations, physiological and biochemical characteristics, genetic characteristics, fatty acids, and polar lipids. The results showed that strain XN-5T differed in many characteristics from the reference strains, including phylogeny of the 16S rRNA gene, growing temperature, hydrolysis of starch and casein, the nitrate reduction reaction, α-galactosidase, α-glucosidase, β-glucosidase, assimilation of L-arabinose, D-mannitol, N-acetyl-glucosamine and potassium gluconate, fermentation of sucrose and arabinose, and polar lipid and fatty acid composition. Based on the polyphasic taxonomy data, we determined that strain XN-5T is a new species of Flavobacterium that we called Flavobacterium pacifica sp. nov. |
| Key words: Caroline Seamounts in the Pacific Ocean Flavobacterium polyphasic taxonomy |