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| 脉红螺Rapana venosa变态过程中自噬与凋亡研究 |
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石璞1,2,3,4,5,6, 宋浩1,2,3,4,6, 杨美洁1,2,3,4,6, 胡志1,2,3,4,5,6, 周骢1,2,3,4,5,6, 李海州7, 张涛1,2,4,6
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1.中国科学院海洋生态与环境科学重点实验室, 山东 青岛 266071;2.青岛海洋科学与技术国家实验室 海洋生态与环境科学功能实验室, 山东 青岛 266237;3.中国科学院海洋大科学研究中心, 山东 青岛 266071;4.中国科学院海洋牧场工程实验室, 山东 青岛 266071;5.中国科学院大学, 北京 100049;6.山东省实验海洋生物学重点实验室, 山东 青岛 266071;7.山东富瀚海洋科技有限公司, 山东 烟台 265100
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| 摘要: |
| 自噬与凋亡是细胞程序性死亡的主要形式,参与昆虫与两栖类动物的变态,并在旧器官的退化中发挥重要作用,但其在软体动物变态中的作用尚不清楚。本文对脉红螺Rapana venosa变态过程中自噬与凋亡的表达特征进行了研究,并通过药理学手段对脉红螺幼虫变态时期的自噬与凋亡进行了诱导和抑制。组织细胞学结果显示,在变态开始后,脉红螺幼虫面盘细胞自噬与凋亡的发生遵循严格的时序性关系,自噬的发生先于凋亡的发生;分子表达定位结果显示自噬与凋亡首先发生在脉红螺幼虫面盘的纤毛细胞中;对脉红螺变态时期进行自噬与凋亡的诱导和抑制后发现,面盘细胞中高水平的自噬会诱导凋亡发生,高水平的凋亡则会抑制自噬的表达。除此之外,脉红螺变态时期自噬与凋亡的诱导有助于脉红螺完成变态。这说明在脉红螺幼虫变态过程中,自噬对面盘进行充分利用后会诱导凋亡产生,自噬与凋亡协同作用使面盘退化。该结果有助于软体动物变态机制的研究,为自噬与凋亡在软体动物中的作用提供了新见解。 |
| 关键词: 自噬 凋亡 脉红螺 变态 |
| DOI：10.11759/hykx20220423003 |
| 分类号: |
| 基金项目:国家自然科学基金项目(32002409,31972814);山东省自然科学基金青年项目(ZR2019BD003);中国博士后科学基金(2019M652498,2021M703248);山东省重点研发计划重大科技创新工程项目(2019JZZY020708);财政部和农业农村部:国家现代农业产业技术体系资助;泰山产业领军人才工程专项经费资助(LJNY201704);烟台市“双百计划”蓝色产业领军人才团队(烟发改蓝色经济〔2018〕30号);青岛海洋科学与技术试点国家实验室海洋生态与环境科学功能实验室创新团队项目(LMEES-CTSP-2018-1) |
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| Autophagy and apoptosis during metamorphosis in Rapana venosa |
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SHI Pu1,2,3,4,5,6, SONG Hao1,2,3,4,6, YANG Mei-jie1,2,3,4,6, HU Zhi1,2,3,4,5,6, ZHOU Cong1,2,3,4,5,6, LI Hai-zhou7, ZHANG Tao1,2,4,6
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1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;4.CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;5.University of Chinese Academy of Sciences, Beijing 100049, China;6.Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao 266071, China;7.Fuhan Ocean Technology Co., Ltd, Yantai 265100, China
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| Abstract: |
| Autophagy and apoptosis, the primary forms of programmed cell death, are involved in insect and amphibian metamorphosis and organ degeneration. However, their mechanisms are poorly understood in mollusks. This study investigates Rapana venosa metamorphosis and the expression patterns of autophagy and apoptosis induced and inhibited by pharmacological methods during metamorphosis. Histological results show that autophagy and apoptosis follow a strict temporal relationship: the expression of autophagy precedes apoptosis. Moreover, molecular expression and localization results indicate that autophagy and apoptosis first occur in ciliated velum cells. A high level of autophagy induces apoptosis, whereas apoptosis inhibits autophagy. In addition, the induction of autophagy and apoptosis help R. venosa complete metamorphosis. This study posits that autophagy induces apoptosis during metamorphosis after fully utilizing the velum. Moreover, autophagy and apoptosis act synergistically to degrade the velum. This study provides a better understanding of R. venosa metamorphosis from the perspective of autophagy and apoptosis. |
| Key words: autophagy apoptosis Rapana venosa metamorphosis |
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