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| 渤、黄海大泷六线鱼耳石微化学特征与群体判别 |
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王英俊1,2,3, 孔晓1,4, 赵文溪1,2,3, 于超勇1,2,3, 王馨1,3, 唐学玺4, 刘洪军1,2,3, 宋爱环1,2,3
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1.山东省海洋科学研究院, 山东 青岛 266104;2.青岛市海洋生物种质资源挖掘与利用工程实验室, 山东 青岛 266104;3.山东省智慧海洋牧场重点实验室, 山东 青岛 266104;4.中国海洋大学 海洋生命学院, 山东 青岛 266003
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| 摘要: |
| 为探究中国近海大泷六线鱼(Hexagrammos otakii)耳石微化学成分特征及群体分化状况,采集了连云港、青岛、威海、烟台、秦皇岛、大连、丹东7个野生群体和1个威海养殖群体的大泷六线鱼样本。利用LA-ICPMS技术测定耳石中的微量元素,以微量元素与钙的比值进行了分析,结果显示大泷六线鱼耳石中,Na/Ca、Sr/Ca、Fe/Ca、Mg/Ca较高,而Mn/Ca、Ba/Ca、Zn/Ca、Li/Ca较低。耳石1龄前元素比值的组间比较(S-N-K检验)结果显示:Na/Ca、Sr/Ca、Fe/Ca在群体间具显著性差异(P<0.05)。耳石边缘区元素与环境中元素比较发现,Li、Mn元素在耳石中富集程度较大,Sr在耳石的中元素含量远远大于水环境中的浓度,说明耳石中的元素沉积与海水中元素并不是简单的线性关系。判别分析结果显示:耳石1龄前元素指纹的判别分析结果表明,大泷六线鱼群体的判别成功率为63.0%,判别正确范围为30.0%~80.0%,耳石边缘区元素的判别正确率为57.1%,判别正确范围20.0%~80.0%,秦皇岛群体有高的判别成功率80.0%。耳石元素指纹可以用于不同大泷六线鱼群体的判别研究。 |
| 关键词: 耳石 微化学元素 指纹 大泷六线鱼(Hexagrammos otakii) 群体判别 激光剥蚀 |
| DOI：10.11759/hykx20220518002 |
| 分类号:Q149 |
| 基金项目:山东省重点研发计划项目(2021LZGC027);中央引导地方科技发展资金项目(YDZX2021024);中国工程科技发展战略山东研究院咨询项目(202103SDYB01) |
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| Microchemical characteristics of otoliths and stock discrimination of Hexagrammos otakii from the Bohai and Yellow Seas |
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WANG Ying-jun1,2,3, KONG Xiao1,4, ZHAO Wen-xi1,2,3, YU Chao-yong1,2,3, WANG Xin1,3, TANG Xue-xi4, LIU Hong-jun1,2,3, SONG Ai-huan1,2,3
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1.Marine Science Research Institute of Shandong Province, Qingdao 266104, China;2.Engineering Laboratory for Exploration and Utilization of Marine Germplasm Resources of Qingdao, Qingdao 266104, China;3.shandonq Key Laboratory of Intelligent Marine Ranch(under preparation), Qingdao 266104, China;4.College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
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| Abstract: |
| Dividing populations is the basis of commercial fish population assessments and scientific management. To investigate the microchemical composition characteristics and group differentiation of Hexagrammos otakii otoliths in offshore China, 7 wild H. otakii populations from Lianyungang, Qingdao, Weihai, Yantai, Qinhuangdao, Dalian, Dandong, and 1 aquaculture population from Weihai were collected. The microchemical elements in the otoliths were determined by laser ablation inductively coupled plasma mass spectrometry, and the ratio of elements to calcium was analyzed. The results showed that the Na/Ca, Sr/Ca, Fe/Ca, and Mg/Ca ratios were higher in otoliths, while the Mn/Ca, Ba/Ca, Zn/Ca, Li/Ca ratios were lower. The difference in elemental content between pre-1 age otoliths and the marginal zone revealed the interannual difference in element deposition. The Sr/Ca ratio in the otoliths showed the marine life history characteristics of H. otakii. Significant differences in the Na/Ca, Sr/Ca, and Fe/Ca ratios were observed among the groups (S-N-K test, P<0.05). After comparing the elements in the otolith marginal zone with the elements in the environment, Li and Mn were highly enriched in the otoliths, and the elemental content of Sr was much higher in the otoliths than that in the water environment, indicating that elemental deposition in the otolith is closely related to seawater. Elemental deposition in otoliths is not a simple linear relationship with the elements in seawater. The results of discriminant analysis of the Na/Ca, Sr/Ca, Mg/Ca, and Mn/Ca element fingerprints of the otoliths before the pre-1 age showed that the success rate of discrimination in the H. otakii populations was 63.0%, and the correct range of discrimination was 30.0%~80.0%. The correct rate of discriminating elements in the marginal zone of the otoliths was 55.8%, and the correct range of discrimination was 40.0%~88.9%. The Qinhuangdao group had the highest discrimination success rate of 80.0%. These results suggest that otolith elemental fingerprints are useful for discriminating different populations of H. otakii. |
| Key words: otolith morphology elemental fingerprint Hexagrammos otakii stock discrimination laser ablation |
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