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| 低温对菲律宾蛤仔热休克蛋白和抗冻蛋白基因表达的影响 |
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张月1,2, 李宁1,2, 黎强1,2, 聂鸿涛1,2, 霍忠明1,2, 闫喜武1,2
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1.大连海洋大学水产与生命学院, 辽宁 大连 116023;2.大连海洋大学 辽宁省贝类良种繁育工程技术研究中心, 辽宁 大连 116023
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| 摘要: |
| 为研究菲律宾蛤仔(Ruditapes philippinarum)“斑马蛤 2 号”在低温胁迫下热休克蛋白和抗冻蛋白基因表达的变化, 将菲律宾蛤仔“斑马蛤 2 号”和南北方野生群体蛤仔急性低温(-1 ℃ )胁迫 9 d, 存活率大小为斑马蛤 2 号(78.08%±2.14%)>大连群体(70.37%±3.70%)>北海群体(48.15%±7.71%)。低温胁迫下, 挑选 5 个蛤仔抗低温相关的基因, 热休克蛋白 70 基因(HSP70), HSC70, CSDE1, Y-box, 抗冻蛋白Ⅱ 型基因(AFPⅡ ), 研究这些基因在 3 个群体菲律宾蛤仔(斑马蛤 2 号、大连群体和北海群体)急性低温胁迫(-1 ℃ )下鳃组织的表达变化。结果显示, 5 个基因在菲律宾蛤仔鳃组织中的时序表达与温度显著相关, 其中 HSP70、 HSC70 在 3 个群体中显著高表达。冷休克蛋白家族的基因 CSDE1、 Y-box 基因只在低温处理的广西群体鳃组织中显著高表达。 3 个群体 AFPⅡ 基因相对表达量在 12 h 显著升高, 达到峰值, 相对表达量斑马蛤 2 号>大连群体>北海群体。不同群体蛤仔在低温胁迫时抗低温相关基因表达模式不同, 斑马蛤 2 号的 HSP70、 HSC70 和 AFPⅡ 表达量及存活率高于 2 个野生群体。 |
| 关键词: 菲律宾蛤仔 群体 低温 存活率 热休克蛋白 抗冻蛋白 |
| DOI：10.11759/hykx20230227004 |
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| 基金项目:国家重点研发计划 (2018YFD0901400); 现代农业产业技术体系建设专项(CARS-49); 辽宁省教育厅项目(LJKZ0701, QL201703) |
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| Effects of low temperature stress on heat shock and antifreeze proteins in Manila clam (Ruditapes philippinarum) |
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ZHANG Yue1,2, LI Ning1,2, LI Qiang1,2, NIE Hongtao1,2, HUO Zhongming1,2, YAN Xiwu1,2
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1.College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;2.Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province, Dalian 116023, China
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| Abstract: |
| Manila clam (Ruditapes philippinarum) is one of the most commercially important aquaculture species in Asia. Three populations of 100 clams were exposed to acute low temperature (-1 ℃ ) stress for 9 days, and the survival rates, in descending order, were the Zebra clam 2 (78.08% ± 2.14%), the Dalian population (70.37% ± 3.70%), and the Beihai population (48.15% ± 7.71%). The expression of HSP70, HSC70, CSDE1, Y-box, and AFPⅡ genes was investigated in these populations (zebra clam 2, Dalian population, and Beihai population) under acute low temperature stress (-1 ℃ ). The results showed that the temporal expression of the five genes in the gill tissue of the Manila clam was significantly correlated with temperature (P<0.05), among which HSP70 and HSC70 were significantly higher in the three populations (P<0.05). The CSDE1 and Y-box genes of the antifreeze protein family were only highly expressed in the gill tissues of the Guangxi population treated with low temperatures. The relative expression of the AFPⅡ gene in the three populations increased significantly, reaching maximum values at highest at 12 h (P<0.05). The relative gene expression was highest in Zebra Clam 2, followed by Zebra Clam 2 and the Beihai population (P<0.05). The expression patterns of genes related to low temperature resistance were different in different populations of clams. The expression levels and survival rates of HSP70, HSC70, and AFPⅡ in Zebra Clam 2 were higher than those in the wild Dalian and Beihai populations. |
| Key words: Ruditapes philippinarum population cold stress survival rate heat shock protein antifreeze protein |