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| 微塑料对四角蛤蜊滤食、呼吸和免疫指标的影响 |
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唐瑞锴1, 杨光1, 孙中伟1, 唐佳伟1, 王鑫1, 马晓娜1,2,3, 冯志华1,2,3
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1.江苏海洋大学 江苏省海洋生物资源与环境重点实验室, 江苏 连云港 222005;2.江苏海洋大学 江苏省海洋生物技术重点实验室, 江苏 连云港 222005;3.江苏海洋大学 江苏省海洋生物产业技术协同创新中心, 江苏 连云港 222005
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| 摘要: |
| 为探讨微塑料对四角蛤蜊生理行为和免疫系统的影响, 研究了不同浓度(1×104 粒/L和1×106 粒/L)和不同粒径(1 μm和80 nm)的聚苯乙烯(PS)暴露对四角蛤蜊滤食率、呼吸率、丙二醛(MDA)浓度和过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)活性的影响。结果表明, 仅1 μm低浓度组对四角蛤蜊滤食率有显著影响; 在高浓度、大粒径微塑料暴露下的四角蛤蜊呼吸率显著上升; SOD和CAT活性均在实验初期显著上升, 实验后期呈下降趋势; ACP的活性在实验前期显著上升, 在后期逐渐下降至与对照组无显著差异; AKP活性在3 d后发生显著性变化, 且低浓度组活性高于高浓度组; MDA浓度显著上升, 并呈现出高浓度组大于低浓度组、小粒径组大于大粒径组的规律。不同浓度、粒径、暴露时间会对各项指标产生影响, 四角蛤蜊鳃中MDA的浓度可作为未来评估海洋微塑料污染的可靠指标。 |
| 关键词: 微塑料 四角蛤蜊 滤食率 呼吸率 免疫系统 |
| DOI：10.11759/hykx20220728005 |
| 分类号:X55;X503.225 |
| 基金项目:江苏省产学研合作项目(BY2020428);江苏高校优势学科建设工程项目;江苏省研究生科研与实践创新计划项目(KYCX20_2882) |
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| Effects of microplastics on filtration, respiratory, and immune indexes of Mactra veneriformis |
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TANG Ruikai1, YANG Guang1, SUN Zhongwei1, TANG Jiawei1, WANG Xin1, MA Xiaona1,2,3, FENG Zhihua1,2,3
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1.Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China;2.Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China;3.Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang 222005, China
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| Abstract: |
| To determine the effects of microplastics on the physiological behavior and immune system activity of Mactra veneriformis, the effects of polystyrene (PS) exposure at different concentrations (1×104 molecules/L and 1×106 molecules/L) and particle sizes (1 μm and 80 nm) on the filtration rate, respiration rate, malondialdehyde (MDA) content and impacts on activities of enzymes such as catalase (CAT), superoxide dismutase (SOD), acid phosphatase (ACP), alkaline phosphatase (AKP) were investigated. The results revealed that only the low concentration group of 1 μm had a significant effect on the filtration rate; the respiration rate was significantly increased under exposure to large particles of polystyrene at high concentrations. The activities of SOD and CAT both increased significantly in the early stages of the experiment and then decreased. ACP activity increased significantly in the early stages and gradually decreased. The changes were not statistically significant. AKP activity changed significantly after 3 days, and the activity in the group exposed to low concentrations was higher than that of the group exposed to high concentrations. Moreover, MDA content significantly increased in the group exposed to high concentrations compared to the group exposed to low concentrations, and MDA content of the group exposed to small polystyrene particles increased compared to the group exposed to large particles. Different concentrations, particle sizes, and exposure times affected multiple physiological indicators. MDA content of M. veneriformis gills can be used as a reliable indicator to assess marine microplastic pollution. |
| Key words: microplastics Mactra veneriformis filtration rate respiration rate immune system |