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| 多功能海藻多糖复合膜的制备及其对UVB诱导的光老化的保护作用 |
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吴雨萌1,2,3, 王晶1,2, 张全斌1,2
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1.中国科学院海洋研究所实验海洋生物学重点实验室, 山东 青岛 266071;2.青岛海洋科学与技术试点国家实验室, 海洋生物与生物技术功能实验室, 山东 青岛 266237;3.中国科学院大学, 北京 100049
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| 摘要: |
| 长期暴露在紫外线B(UVB)的照射下会导致皮肤光老化。海藻多糖是一类结构复杂的海洋酸性多糖, 具有抗氧化、抗炎以及免疫调节等多种生物活性。其寡糖因分子量低、水溶性好等特点, 具有更好的生物活性和应用潜力。本文以硫酸化半乳岩藻聚糖(DF)和褐藻胶寡糖(AOS)为活性成分, κ-卡拉胶(κ-Car)为基质制备了多功能海藻多糖复合膜——FAC膜, 通过理化性质表征以及体内实验探究其对UVB诱导的皮肤光老化的保护作用。理化测定结果表明, FAC膜具有良好的吸湿和保湿性, 在不同pH环境下具有缓释DF的效果。FAC膜在去离子水中浸泡3 min后, 具有较好的黏弹性。体内实验结果表明, FAC膜处理可提高皮肤含水量, 减少UVB诱导引起的皮肤的表皮层和真皮层厚度的增加; 提高皮肤过氧化氢酶(CAT)和超氧化物歧化酶(SOD)浓度; 降低促炎症因子白介素-6(IL-6)和肿瘤坏死因子(TNF-α)的表达; 提高胶原蛋白特征氨基酸酶羟脯氨酸(HyP)的浓度以及有效降低胶原蛋白降解酶基质——金属蛋白酶-3(MMP-3)的表达。这些结果表明, FAC膜具有保湿、缓释和便于使用等良好理化性质以及修复皮肤屏障、抗氧化、抗炎和抑制胶原蛋白降解的良好生物活性, 可以作为预防和治疗皮肤光老化的制剂。 |
| 关键词: 光老化 硫酸化半乳岩藻聚糖 褐藻胶寡糖 κ-卡拉胶 多功能复合膜 |
| DOI：10.11759/hykx20230410002 |
| 分类号:R758.14 |
| 基金项目:山东省自然科学基金(ZR2022MD007);福建省科技项目(2022T3024) |
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| Preparation of a multifunctional seaweed polysaccharide-based composite film and its protective effects against UVB-induced photoaging |
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WU Yumeng1,2,3, WANG Jing1,2, ZHANG Quanbin1,2
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1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.Marine Biology and Biotechnology Laboratory, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China;3.University of Chinese Academy of Sciences, Beijing 100049, China
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| Abstract: |
| Long-term exposure to ultraviolet B (UVB) radiation causes photoaging of the skin. Seaweed-derived polysaccharides are a class of structurally complex, acidic, marine polysaccharides with various biological activities such as antioxidant, anti-inflammatory, and immunomodulatory effects. Their oligosaccharides have better bioactivity and application potential due to low molecular weight and good water solubility. In this paper, a multifunctional seaweed polysaccharide-based composite film, the FAC film, was prepared using sulfated galactofucan polysaccharides (DF) and oligosaccharides sodium alginate (AOS) as active ingredients, and κ-carrageenan (κ-Car) as the matrix. Next, their protective effects against UVB-induced skin photoaging were investigated via physicochemical property characterization and in vivo experiments. The results of the physicochemical measurements showed that the film had good hygroscopic and moisturizing properties and a slow-release DF effect in different pH environments. The film exhibited good viscoelasticity after soaking in deionized water for 3 mins. The results of the in vivo experiments revealed that treatment with FAC film enhanced the skin water percentage and reduced UVB-induced thickening of the epidermis and dermis of the skin; elevated skin catalase (CAT) and superoxide dismutase (SOD) concentrations; suppressed expression of the pro-inflammatory factors interleukin-6 (IL-6) and tumor necrosis factor (TNF-α); increased levels of the collagen characteristic amino acid hydroxyproline (HyP); and effectively reduced the expression of the collagen degrading enzyme substrate, metalloproteinase-3 (MMP-3). These results suggested that the FAC film has excellent physicochemical properties such as moisturization, slower release, and ease of application, as well as appreciable biological activities of skin barrier repair and antioxidant, anti-inflammatory, and collagen degradation inhibition effects. Thus, it could be utilized as an agent for the prevention and treatment of skin photoaging. |
| Key words: photoaging sulfated galactofucan polysaccharides oligosaccharides sodium alginate κ-carrageenan multifunctional composite film |