| 摘要: |
| 为了明确文蛤游离氨基酸的组成、含量及其风味特性, 本研究以文蛤的11个全同胞家系为研究对象, 利用氨基酸自动分析仪测定文蛤足组织中游离氨基酸的种类和含量, 通过主成分分析、TOPSIS分析进行综合评价。结果显示, 文蛤家系游离氨基酸总量均在473~816 mg/100g, 均值为638 mg/100g。呈鲜味氨基酸含量为270~570 mg/100g, 占总含量的56.0%~70.8%, 是最主要的呈味氨基酸。15种游离氨基酸通过主成分分析提取到2个主成分, 其累积方差贡献率为89.54%, 可代表文蛤中游离氨基酸的综合信息。游离氨基酸T值较高的家系依次是22A8、22A5、22A1和22A6, 与TOPSIS分析结果一致。结果表明上述4个文蛤家系可作为游离氨基酸含量综合性状突出的优良种质资源用于文蛤的选育工作。 |
| 关键词: 文蛤(Meretrix petechialis) 游离氨基酸含量 主成分分析 TOPSIS分析 |
| DOI：10.11759/hykx20240304002 |
| 分类号:Q348 |
| 基金项目:山东省重点研发计划(农业良种工程)项目(2021LZGC029)、现代农业产业技术体系(CARS-49)、山东德和明兴生物科技有限公司泰山产业领军人才项目、中国科学院战略性先导科技专项(XDA24030304)和山东省自然科学基金(ZR2020MD089) |
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| Comprehensive evaluation of different free amino acid content in clams Meretrix petechialis |
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TIAN Yanmin1,2, WANG Hongxia1, LIU Baozhong1,2
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1.CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China
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| Abstract: |
| This study evaluated 11 different full-sib families of the clam Meretrix petechialis to elucidate the composition, content, and flavor characteristics of free amino acids. The type and content of free amino acids in clam foot tissues were determined using an automatic amino acid analyzer. Principal component analysis and TOPSIS (Technique for Order Preference by Similarity to an Ideal Solution) analysis methods were used to comprehensively evaluate 11 families. The total free amino acid content in clam families ranged from 473 to 816 mg/100 g, with an average of 638 mg/100 g. Among the clam families, the fresh flavor amino acid content, ranging from 270 to 570 mg/100 g, accounted for 56.0% to 70.8% of the total content, making it the most important flavor amino acid in clams. Fifteen free amino acids were extracted using the principal component analysis, and were divided into two principal components, with a cumulative variance contribution rate of 89.54%, representing comprehensive information on free amino acids in clams. Families with higher T-values in comprehensive evaluation were 22A8, 22A5, 22A1, and 22A6, consistent with the TOPSIS analysis. The results demonstrated that four clam families can be used as excellent germplasm resources with outstanding comprehensive characteristics of free amino acid content for clam breeding. |
| Key words: Meretrix petechialis free amino acid content principal component analysis TOPSIS analysis |
