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| 硬壳蛤(Mercenaria mercenaria)生长性状转录组差异分析 |
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梁斌1,2,3,4,5,6, 杨美洁1,2,3,4,5,6, 马兴浩7, 石璞1,2,3,4,5,6, 周骢1,2,3,4,5,6, 曲锦鹏4, 赖长苹8, 张涛1,2,4,6
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1.中国科学院海洋生态与环境科学重点实验室, 山东 青岛 266071;2.青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室, 山东 青岛 266237;3.中国科学院海洋大科学研究中心, 山东 青岛 266071;4.中国科学院海洋牧场工程实验室, 山东 青岛 266071;5.中国科学院大学 北京 100049;6.山东省实验海洋生物学重点实验室, 山东 青岛 266071;7.寿光市海洋渔业发展中心, 山东 寿光 262700;8.连云港蓝碳海洋科技有限公司, 江苏 连云港 222066
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| 摘要: |
| 经济物种的生长性状直接影响其经济产值。本研究以生长速度具有显著差异的同龄硬壳蛤(Mercenaria mercenaria)成贝为研究对象, 利用转录组测序探究生长快慢个体组间基因表达差异, 并探寻可能影响硬壳蛤生长速度的关键基因。结果显示, 快速生长与缓慢生长的硬壳蛤群体间共有1 590个差异基因, 通过对差异基因进行功能富集分析发现, 这些差异基因主要在PPAR信号转导、免疫、消化等相关通路上显著富集。编码蛋白磷酸酶调节亚基和过氧化物酶体酰基辅酶A的基因可能在PPAR信号通路中影响硬壳蛤的糖代谢、物质传递等生命活动过程, 进而导致不同硬壳蛤生长速度的差异。这些基因表达量高的个体可能具有较强的细胞内的代谢和物质传递能力, 因此生长速度较快; 此外, 脂类消化代谢相关基因3-酮酰基-CoA硫解酶在生长快速的硬壳蛤中表达显著下调, 这表明生长快速的硬壳蛤可能脂肪酸降解速度较慢, 导致更多脂肪酸在生物体内积累; 另一方面, 多糖消化代谢相关通路中葡萄糖差向异构酶在生长快速的硬壳蛤中表达显著上调, 过氧化物蛋白酶、组织蛋白酶和蛋白磷酸酶等免疫相关基因在生长快速的硬壳蛤中表达显著上调。这表明生长快速的硬壳蛤群体可能具有更强的消化能力和免疫能力, 从而保证了其更快的生长速度。本研究结果为硬壳蛤的生长性状遗传机制提供了新的见解, 为下一步的育种工作提供了理论依据。 |
| 关键词: 硬壳蛤 生长性状 转录组 差异基因 |
| DOI：10.11759/hykx20240407002 |
| 分类号:S917.4 |
| 基金项目:山东省重点研发计划(重大科技创新工程)项目(2023CXGC010411);天津市科委项目(20YFZCSN00240);天津市农委项目(202103010);连云港市连云区“山海英才计划”领军人才项目;连云港市花果山英才“双创计划”(创业类)项目;现代农业产业技术体系专项资金项目(CARS-49);广西重点研发计划项目(2021AB34014) |
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| Transcriptome analysis of growth trait differences in Mercenaria mercenaria |
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LIANG Bin1,2,3,4,5,6, YANG Meijie1,2,3,4,5,6, Ma Xinghao7, SHI Pu1,2,3,4,5,6, ZHOU Cong1,2,3,4,5,6, QU Jinpeng4, LAI Changping8, ZHANG Tao1,2,4,6
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1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;4.CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;5.University of Chinese Academy of Sciences, Beijing 100049, China;6.Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao 266071, China;7.Shouguang Marine Fisheries Development Center, Shouguang 262700, China;8.Lianyungang Blue Carbon Marine Technology Co., Ltd., Lianyungang 222066, China
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| Abstract: |
| The growth traits of economically important species directly affect their economic value. In this study, we focused on cohorts of the hard clam (Mercenaria mercenaria) with significant differences in growth rates. Transcriptome sequencing was performed to investigate the intergroup gene expression differences related to fast and slow growth and to explore key genes that may influence the growth rate of hard clams. The results revealed 1 590 differentially expressed genes between fast- and slow-growth groups of hard clams. Functional enrichment analysis of these differentially expressed genes indicated significant enrichment in pathways related to PPAR signal transduction, immunity, and digestion. Genes encoding protein phosphatase regulatory subunits and peroxisome proliferator-activated receptor gamma coactivator 1-alpha may affect glucose metabolism and substance transfer processes in the PPAR signaling pathway, thereby contributing to differences in growth rates among hard clams. Individuals with higher expression levels of these genes may possess stronger metabolic and substance transfer capabilities, which result in faster growth rates. In addition, genes related to lipid digestion and metabolism, such as 3-ketoacyl-CoA thiolase, were significantly downregulated in fast-growing hard clams, which suggests a slower rate of fatty acid degradation and a higher accumulation of fatty acids in these clams. Conversely, glucose-6-phosphate isomerase in pathways related to polysaccharide digestion and metabolism, and immune-related genes, such as peroxiredoxins, tissue proteases, and protein phosphatases, were significantly upregulated in fast-growing hard clams. This result indicates that fast-growing hard clam cohorts may possess stronger digestive and immune capabilities, thereby ensuring their faster growth rates. The findings of this study provide new insights into the genetic mechanis. |
| Key words: Mercenaria mercenaria Growth traits Transcriptome Differential gene |
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