| 摘要: |
| 虾壳是目前甲壳素的最主要来源。作为一种环保高效的方法, 采用脱钙、脱蛋白2种菌株分别作用的两步发酵法已被应用于从虾壳中提取甲壳素。首先, 本文从不同来源分离得到产酸菌株植物乳杆菌(Lactiplantibacillus plantarum)及产蛋白酶菌株铜绿假单胞菌(Pseudomonas aeruginosa), 进一步优化发酵条件, 在最佳条件下采用两步发酵提取虾壳中的甲壳素, 进一步采用10% H2O2脱色, 其中先脱蛋白后脱钙的顺序综合表现出的钙和蛋白质的去除率更高, 所得脱钙率及脱蛋白率分别为(96.92±0.07)%及(78.46±1.29)%。采用傅里叶变换红外光谱(FTIR)、X射线衍射(XRD)及扫描电子显微镜(SEM)对所得产物进行表征, 发现其与商用甲壳素结构相似度高, 结晶指数为78.70%, 微观结构表现为纤维状并带有孔隙。以上结果表明, 利用这2种微生物进行两步发酵法可获得较高纯度的甲壳素。本研究为利用微生物法有效提取虾壳来源的甲壳素提供了参考。 |
| 关键词: Lactiplantibacillus plantarum Pseudomonas aeruginosa 微生物法 甲壳素提取 |
| DOI：10.11759/hykx20240228001 |
| 分类号:P746 |
| 基金项目:山东省重点研发计划(重大科技创新工程)项目(2022CXGC020413);国家自然科学基金(42276097;41976096);山东省优青(ZR2022YQ37) |
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| Green preparation of chitin from shrimp shells by two-step fermentation using microbiological method |
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ZHOU Yi1,2, YANG Haoyue1, XING Ronge1, LIU Song1, YU Huahua1, LI Kecheng1, LI Pengcheng1
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1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China
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| Abstract: |
| Shrimp shells are currently the most important source of chitin. An environmentally friendly and efficient two-step fermentation method, i.e., demineralization (DM) and deproteinization (DP), has been employed to extract chitin from shrimp shells. In this study, an acid-producing strain (Lactiplantibacillus plantarum) and a protease-producing strain (Pseudomonas aeruginosa) were isolated from different sources and used to extract chitin from shrimp shells using a two-step fermentation method under optimal conditions, followed by decolorization with 10% H2O2. The sequence of DP followed by DM demonstrated superior calcium and protein removal, achieving 96.92%±0.07% and 78.46%±1.29%, respectively. Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy were used to characterize the product, all of which indicated a high degree of structural similarity to commercial chitin. The crystallization index was 78.70%, and the microstructure revealed a fibrous structure with pores. These results indicated that the two-step fermentation method yields chitin of higher purity. This study provides a valuable reference for the effective extraction of chitin from shrimp shells using microbiological methods. |
| Key words: Lactiplantibacillus plantarum Pseudomonas aeruginosa microbiological method chitin extraction |
