| 摘要: |
| 深海环境动态变化下的微生物研究需要长周期大量时序样品。本文提出一种基于卷筒缠放的多滤膜深海悬浮体低污染抽滤采样装置,可实现360次有效直径60 mm的海水样品过滤。同时设计了滤膜盖层、滤膜以及滤膜固定层的多层滤膜结构来降低非采样位置和样品保存位置的污染。对装置的机械结构与控制系统进行设计。滤膜滤水试验的结果表明PES(0.45 μm, 1 μm)以及PTFE(0.22 μm)的滤膜均具有较好的过水性能。采样装置在深海高压模拟系统中完成了各项取样动作, 并在中国南海1 500 m海域6个不同站点完成了采样试验, 成功获取了甲烷渗漏区的悬浮体样品, 为海底甲烷生态系统中碳的固定、传输和埋藏过程研究提供了技术支撑。 |
| 关键词: 海洋观测 深海悬浮体 深海微生物 原位抽滤 多滤膜 |
| DOI：10.11759/hykx20241212001 |
| 分类号:P715 |
| 基金项目:国家自然科学基金面上资助项目(41977269) |
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| Design and experimental study of an in situ sequential filtration sampling device for deep-sea suspension |
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JIN Shidi1, ZHANG Pingping1, LIU Ying1, CHEN Jiawang2
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1.Ocean College, Zhejiang University, Zhoushan 316021, China;2.Donghai Laboratory, Zhoushan 316021, China
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| Abstract: |
| The study of microorganisms under the dynamic change of the deep-sea environment requires numerous long-period time series samples. In this paper, a low-pollution extraction and sampling device based on a multifilter membrane capable of filtering seawater samples with an effective diameter of 60 mm 360 times is proposed for deep-sea suspension. Simultaneously, the multilayer filter membrane structure of the filter membrane cover, filter membrane, and filter membrane fixed layer is designed to reduce the contamination of non-sampling and sample storage sites. The mechanical structure and control system of the device are designed. Water filtration test results showed that PES (Polyethersulfone, 0.45 μm, 1 μm) and PTFE (Polytetrafluoroethylene, 0.22 μm) had better water filtration performance. The sampling device completed various sampling actions in the deep-sea high-pressure simulation system, completed sampling tests at six stations in a 1500 m sea area of the South China Sea, and successfully obtained suspended samples in the methane leakage area, providing technical support for research on the carbon fixation, transport, and burial process in the submarine methane ecosystem. |
| Key words: ocean observation deep sea suspension deep-sea microorganism in situ filtration multifilter membrane |
