| 摘要: |
| 采用平板分离法,从黄海海域分离到一株聚磷菌,编号为YSR-3,对其进行了部分生理生化特性以及分子特性的研究。结果表明,YSR-3菌体呈杆状,大小为3.5μm×1μm,革兰氏染色阴性,好氧生长;显微镜下观察能运动。最适培养条件为:氯化钠浓度3%,生长温度为24℃,起始pH值为6.5。该菌能在细胞内累积多磷酸盐形成异染粒。16SrDNA鉴定结果表明,YSR-3属于γ-变形菌纲、盐单胞菌属(Halomonas)。与盐单胞菌属中的其他已知种比较,YSR-3具有其特性:对氯霉素和卡那霉素敏感、淀粉水解呈阳性、反硝化和几丁质降解呈阴性、能将葡萄糖作为唯一碳源和能源。 |
| 关键词: 聚磷菌 多磷酸盐 盐单胞菌 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目,40376048号;国家重点基础研究专项经费资助项目,2006CB400600号,G19990437号。 |
附件 |
|
| ISOLATION AND IDENTIFICATION OF A MARINE POLYPHOSPHATE-ACCUMULATING BACTERIUM YSR-3 |
|
REN Shi-Ying1,2, WANG Zi-Feng1,2, XIAO Tian1, Serge Nitsche3
|
|
1.Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071;2.Graduate School, Chinese Academy of Sciences, Beijing, 100039;3.Centre de Recherche en Matière Condensée et Nanosciences, UPR7251, CNRS,Campus de Luminy, Case 913, 13288 Marseille Cedex 9, France
|
| Abstract: |
| Strain YSR-3, a polyphosphate-accumulating bacterium, was recently isolated from the Yellow Sea in August, 2004. The cell is rod-shaped, 3.5μm × 1μm, Gram negative, aerobic, and able to move. In certain stages of its growth, the cell can accumulate polyphosphate to form metachromatic granules. Based on the 16S rDNA analysis, Strain YSR-3 belongs to genus Halomonas, γ-Proteobacteria. In addition to some common traits of Halomonas, the cell features its own special characters unlike other known Halomonas species. It is susceptible to some antibiotics, such as Kn and Cl. The activity of amylase is positive but that of denitrification is negative. The cell can use glucose as a sole source of carbon for the energy. The optimal condition for the cell cultivation is 30 in salinity, 24°C, 180r/min, and initial pH at 6.5.
Strain YSR-3 be longs to halophilic genus Halomonas, and can accumulate polyphosphate in its body. This trait has not so far reported in other strains of genus Halomonas, and this polyphosphate-accumulating is important in the research of phosphorus circulation in oceans, for being a potential actor for biologically removing phosphorus in seawater. Studying the formation and biomineralization of intracellular phosphorus granules in strain YSR-3 would effectively help establishing a biological treatment procedure of phosphorus removal in marine environment. |
| Key words: Polyphosphate-accumulating bacterium, Polyphosphate, Halomonas |
