| 摘要: |
| 利用ISSR 标记对牙鲆♀与石鲽♂单对杂交亲本及其子一代(全同胞家系)的分离方式进行了研究。结果表明, 8 个ISSR 引物共扩增出181 个标记, 其中116 个为多态性, 占总数的64.09%; 标记在F1 代呈三种分离方式：符合孟德尔分离比例、偏离孟德尔分离比例和异常分离。符合孟德尔分离比例的情况包括：不分离(代表了亲本基因型的5 种组合：AA×AA、AA×Aa、Aa×AA、AA×aa、aa×AA); 1:1 分离(代表了亲本基因型的2 种组合：Aa×aa、aa×Aa); 3:
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| 关键词: 单对杂交, 牙鲆, 石鲽, ISSR 标记, 分离方式, 连锁图谱 |
| DOI：10.11693/hyhz200905016016 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目, 30671624 号; 国家“863”项目资助, 2006AA10A404 号; 鲁东大学校人才基金资助, LY20083304 号 |
附件 |
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| THE SEGREGATION OF ISSR MARKERS IN THE F1 PROGENY OF INTERSPECIES CROSSBREED BETWEEN PARALICHTHYS OLIVACEUS AND KAREIUS BICOLORATUS |
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XU Jian-Peng1, ZHANG Quan-Qi2, WANG Zhi-Gang2, QI Jie2, WANG Xu-Bo2
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1.Key Laboratory of Marine Genetics and Gene Resource Exploitation, Ministry of Education, College of Marine Life Science, Ocean University of China, College of Life Science, Ludong University;2.Key Laboratory of Marine Genetics and Gene Resource Exploitation, Ministry of Education, College of Marine Life Science, Ocean University of China
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| Abstract: |
| The segregation pattern of ISSR markers in the F1 of interspecies cross between Paralichthys olivaceus and Kareius bicoloratus was studied. Eight primers yielded 181 markers, of which 116 (64.09%) were polymorphic. The segregation showed three patterns: Mendelian, deviated Mendelian, and abnormal. The Mendelian segregation included three types: (1) non-segregation that reflects five genotypic combinations of the parents: AA×AA, AA×Aa, Aa×AA, AA×aa, and aa×AA; (2) 1︰1 segregation, reflecting two genotypic combination of the parents, Aa×aa and aa×Aa; (3) 3︰1 segregation, reflecting one genotypic combination of the parents, Aa×Aa. The deviated pattern includes two types: polymorphic in the parents and deviated from 1︰1 ratio in the progenies and the appeared in the parents and deviated from 3︰1 ratio in progenies. The abnormal pattern includes two types: absent in the parents but present in the progenies, and present in the parents but absent in the progenies. The percentages and amounts of the three patterns were 82.87%, 150; 11.05%, 20; and 6.08%, 11; respectively. In addition, 85.33% of the markers presenting in both the parents did not segregate and 28.57%—35.85% of the markers presenting in only one parent showed normal segregation. The above results could provide certain theoretical basis for further work to the construction of linkage map of P. olivaceus and K. bicoloratus.
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| Key words: Tairmating, Paralichthys olivaceus, Kareius bicoloratus, ISSR markers, Segregation pattern, Linkage map |
