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引用本文:杨佰娟,郑 立,陈军辉,臧家业,王小如,黎先春.黄、渤海漂移浒苔(Enteromorpha prolifera)脂肪酸组成及聚类分析的研究.海洋与湖沼,2009,40(5):627-632.
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黄、渤海漂移浒苔(Enteromorpha prolifera)脂肪酸组成及聚类分析的研究
杨佰娟1, 郑 立1, 陈军辉1, 臧家业1, 王小如2, 黎先春1
1.国家海洋局第一海洋研究所海洋生态研究中心;2.国家海洋局第一海洋研究所海洋生态研究中心,厦门大学化学化工学院化学系 现代分析科学教育部重点实验室
摘要:
于2008 年7 月在黄、渤海区域采集了17 个漂移浒苔样品, 结合3 个本地种, 对其脂肪酸组成进行了GC-MS 分析。结果表明, 所有浒苔中优势脂肪酸为十六碳酸, 相对含量在27.82%—43.27%之间。藻体中还含有丰富的PUFA, 主要为9,12,15-十八碳三烯酸、6,9,12-十八碳三烯酸、9,12-十八碳二烯酸、8,11,14-二十碳三烯酸, 另外还含有EPA 不饱和脂肪酸。以欧式距离平方为距离测量技术, 类间距用平均链锁法, 对20 个不同地理位置的浒苔样品进行聚类分析, 结果分为4 大类
关键词:  浒苔, 脂肪酸, 聚类分析
DOI:10.11693/hyhz200905017017
分类号:
基金项目:海洋公益性行业科研专项, 200805039 号; 国家海洋局第一海洋研究所基本科研业务费专项资金项目, 2008G47 号、2008T32 号;国家自然科学基金资助项目, 20602009 号、40776098 号; 海洋863 项目, 2007AA09Z435 号
附件
CLUSTER ANALYSIS ON FATTY ACID COMPOSITION OF ENTEROMORPHA PROLIFERA OFF NORTHERN CHINA COAST
YANG Bai-Juan1, ZHENG Li1, CHEN Jun-Hui1, ZANG Jia-Ye1, WANG Xiao-Ru2, FRANK S. C. Lee1
1.Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration;2.Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Department of Chemistry and the Key Laboratory of Analytical Science of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen
Abstract:
Fatty acids composition of 20 Enteromorpha prolifera samples from the Yellow Sea and the Bohai Sea were determined with GC-MS. The result indicates that C16 are the predominant fatty acid, taking 27.82% to 43.27%. C18:2 (cis, cis-9,12)(n-6), C18:3 (gamma) (all cis-6,9,12)(n-6), C18:3 (alpha) (all cis-9,12,15)(n-3) and EPA are major polyunsaturated fatty acids (PUFAs) in E. prolifera. The samples were numerically classified in fatty acid composition using Squared Euclidean distance and between-group linkage method as clustering algrithm. The 20 samples were divided into four groups by cluster analysis. All the floating E. prolifera samples are placed in Group I, and other native species in Qingdao and Lianyungang are classified into other three groups. The floating E. prolifera that bloomed in Qingdao coastal area in 2008 were not orininated from local native species but from the open sea. Hierarchical cluster analysis was proved to be a good method of quantitive evaluation and classification of marine green algae.
Key words:  Enteromorpha prolifera, Fatty acids, Cluster annalysis
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