引用本文:	田鑫江,吴宁,黎中宝,林小云,张志红,陈晓姣,陈丽娜.6种鳗鲡(Anguilla)线粒体DNA COⅡ基因序列分析及其系统发育.海洋与湖沼,2011,42(2):279-283.

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*6种鳗鲡(Anguilla)线粒体DNA COⅡ基因序列分析及其系统发育*
田鑫江^1,2, 吴宁^1,2, 黎中宝^1,2, 林小云^1,2, 张志红^1,2, 陈晓姣^1,2, 陈丽娜^1,2
1.集美大学水产学院;2.集美大学水产生物技术研究所

摘要:

采用基因测序的方法, 对 6 种鳗鲡的 COⅡ基因片段进行了 PCR 扩增和测序。结果表明, 6种鳗鲡 COⅡ基因片段的长度都为 412bp, 4 种碱基组成非常相似, 并且 A+T 的含量都大于 G+C 的含量。 6 种鳗鲡 COⅡ基因片段核苷酸序列之间有 46bp 的差异, 其中有 6 处发生碱基颠换, 40 处发生碱基转换。非洲鳗鲡(Anguilla mossambic)和日本鳗鲡(A. japonica)COⅡ基因片段核苷酸序列差异最大为27bp, 同源性为 93.45%, 而日本鳗鲡和菲律宾鳗鲡(A. mormorata)COⅡ基因片段核苷酸序列差异最小为 5bp, 同源性为 98.79%。

关键词: 鳗鲡, mtDNA COⅡ基因, 序列差异, 颠换, 转换

DOI：10.11693/hyhz201102018018

分类号:

基金项目:公益性行业(农业)科研专项经费项目, “鳗鲡种质鉴定与新品种引进”, nyhyzx07-043-08 号; 集美大学创新团队建设项目, 集大人(2007)17 号

附件

SEQUENCING ANALYSIS OF THE mtDNA COⅡGENE FRAGMENTS OF SIX ANGUILLA SPECIES

TIAN Xin-Jiang^1,2, WU Ning^1,2, LI Zhong-Bao^1,2, LIN Xiao-Yun^1,2, ZHANG Zhi-Hong^1,2, CHEN Xiao-Jiao^1,2, CHEN Li-Na^1,2

1.Fisheries College, Jimei University;2.Institute of Aquaculture Biotechnology, Jimei University

Abstract:

MtDNA COⅡgene fragments of six Anguilla species were amplified using the PCR method, and the prod-ucts were purified and sequenced. The results showed that all the mtDNA COⅡgene fragments had 412bp with similar base composition, which had greater amount of the A+T pairs than the G+C pairs. The difference among the six mtDNA COⅡgenes was 46bp, including 6 base transversions and 40 base transitions. The difference between the mtDNA COⅡ genes of A. mossambica and A. japonica was 27bp with 93.45% homology; however the difference between those of A. japonica and A. mormorata was 5bp with 98.79% homology.

Key words: Anguilla, mtDNA COⅡgene, Sequence difference, Transversion, Transition