引用本文:	杨季芳,郭卢云,陈福生,王海丽.2 株南极海洋寡营养细菌(Alteromonas stellipolaris)脂多糖对三疣梭子蟹(Portunus trituberculatus) 非特异性免疫活性的影响.海洋与湖沼,2011,42(2):294-299.

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*2 株南极海洋寡营养细菌(Alteromonas stellipolaris)脂多糖对三疣梭子蟹(Portunus trituberculatus) 非特异性免疫活性的影响*
杨季芳^1,2,3, 郭卢云^1,2,3, 陈福生^1,2,3, 王海丽^2,3
1.华中农业大学食品科学技术学院;2.宁波市微生物与环境工程重点实验室;3.浙江万里学院生物与环境学院

摘要:

从 2 株南极海洋寡营养细菌(Alteromonas stellipolaris, 菌株 AT82 和 AT52)中提取脂多糖(Lipopolysaccharide, LPS), 分不同处理组免疫注射三疣梭子蟹, 在免疫后第 3、6、9、12 天分别检测梭子蟹血清的抗菌活力、溶菌酶活力、超氧化物歧化酶活力、酚氧化酶活力和过氧化物酶活力等非特异性免疫指标的变化。结果表明, 自 2 株南极寡营养细菌提取的脂多糖均能显著提高梭子蟹血清中的抗菌活力、溶菌酶活力、超氧化物歧化酶活力、酚氧化酶活力和过氧化物酶活力等指标(P<0.05)。各种酶活力出现峰值的时间不同, 且 AT82脂多糖注射组比 AT52脂多糖注射组的非特异性免疫增强效果更好。上述物质表现的体内免疫作用机理和最佳免疫剂量有待于进一步研究。

关键词: 南极海洋寡营养细菌, 脂多糖, 三疣梭子蟹, 非特异性免疫

DOI：10.11693/hyhz201102021021

分类号:

基金项目:国家科技部国际合作重点资助项目, 2007DFA21300号; 中德政府海洋科学双边合作项目, BMBF CHN 00/19号; 宁波市科技局重大招标项目, 2007C11002 号; 浙江省教育厅项目, Y200803277 号

附件

THE EFFECTS OF LIPOPOLYSACCHARIDES FROM TWO STRAINS OF OLIGOTROPHIC BACTERIA FROM ANTARCTIC OCEAN ALTEROMONAS STELLIPOLARIS ON NON-SPECIFIC IMMUNITY OF THE SWIMMING CRAB PORTUNUS TRITUBERCULATUS

YANG Ji-Fang^1,2,3, GUO Lu-Yun^1,2,3, CHEN Fu-Sheng^1,2,3, WANG Hai-Li^2,3

1.College of Foods Science & Technology, Huazhong Agricultural University;2.Municipal Key Laboratory of Microorganism and Environmental Engineering;3.Faculty of Biological & Environmental Science of Zhejiang Wanli University

Abstract:

The indices of non-specific immunity were measured in 3, 6, 9, 12 days after Portunus trituberculatus was treated with lipopolysaccharides (LPS) extracted from two Antarctic ultramicro-bacteria Alteromonas stellipolaris, AT52 and AT82. The results showed those LPS were able to improve the activities of anti-bacteria, lysozme (LSZ), phenoloxidase (PO), superoxide dismutase (SOD), and peroxidase (POD) significantly (P<0.05). A variety of enzyme activities reached maximum levels at different days, and AT82 LPS injection group was found better than those of AT52 LPS injection group. Our studies suggested LPS of Antarctic ultramicro-bacteria is a desirable potential immunologic stimulant for P. trituberculatus to fight for bacterial diseases. Future studies are needed to understand the mechanism of the immunity response and the best dosage for these LPS.

Key words: Antarctic seawater oligotrophy bacterium, Lipopolysaccharide, Portunus trituberculatus, Non-specific immunity