引用本文:	徐大伦,颜伟华,杨文鸽,何斌辉,丁宁,陈丹,顾玲芳.响应面法优化双髻鲨(Sphyrna zygaena)肌肉盐溶蛋白的提取和凝胶形成条件.海洋与湖沼,2011,42(2):289-293.

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*响应面法优化双髻鲨(Sphyrna zygaena)肌肉盐溶蛋白的提取和凝胶形成条件*
徐大伦, 颜伟华, 杨文鸽, 何斌辉, 丁宁, 陈丹, 顾玲芳
宁波大学应用海洋生物技术教育部重点实验室

摘要:

采用响应面分析法, 以双髻鲨肉盐溶蛋白凝胶保水性为依据, 对盐溶蛋白的提取和凝胶形成条件 NaCl 浓度、pH 和低温加热时间进行优化。结果表明: (1) 根据中心组合试验设计, 采用三因素三水平的响应面分析法, 建立了双髻鲨肉盐溶蛋白凝胶保水性与其提取条件的数学模型: 保水性(%) = 65.440 + 10.868X₁ + 1.835X₂－0.502X₃－4.647X₁² + 0.121X₁X₂－0.415X₁X₃－0.856X₂² + 0.487X₂X₃－1.789X₃², 其中NaCl浓度和pH对双髻鲨肉盐溶蛋白凝胶的保水性影响极其显著, 低温加热时间对保水性影响不显著; (2) 在分析各因素显著性及其交互作用的基础上, 得出盐溶蛋白提取和凝胶形成的适宜条件为: NaCl浓度为0.90mol/L, 提取溶液的pH = 7.0, 低温加热时间为35min, 此时盐溶蛋白凝胶的保水性达到 72%, 与模型预测值基本相符。

关键词: 双髻鲨, 盐溶蛋白, 凝胶保水性, 响应面法

DOI：10.11693/hyhz201102020020

分类号:

基金项目:国家“十一五”科技支撑计划重大项目, 2008BAD94B05 号; 宁波市自然科学基金项目, 2010A610013 号; 浙江省大学生科技创新基金项目, 2009—2010

附件

RESPONSE SURFACE OPTIMIZATION OF CONDITIONS FOR EXTRACTION AND GEL PREPARATION OF SALT-SOLUBLE PROTEINS FROM SPHYRNA ZYGAENA MUSCLE

XU Da-Lun, YAN Wei-Hua, YANG Wen-Ge, HE Bin-Hui, DING Ning, CHEN Dan, GU Ling-Fang

Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University

Abstract:

Based on the Box-Benhnken experimental design with 3 factors and 3 levels, the effects of NaCl concentration (X₁), pH (X₂) and low-temperature heating time (X₃) on the water holding capacity (WHC) of the salt-soluble protein gel from Sphyrna zygaena muscle were studied and optimized by the respond surface methodology (RSM). The correlation between the WHC of the salt-soluble protein gel and its extractive conditions was WHC(%) = 65.440 + 10.868X₁ + 1.835X₂-0.502X₃-4.647X₁² + 0.121X₁X₂-0.415X1X₃-0.856X₂² + 0.487X_{?2X₃-1.789X₃². The effects of NaCl concentration and pH on the WHC of the salt-soluble protein gel were extremely significant; but the low-temperature heating time was not. Response surface and contour were graphed with the WHC as the response value. The optimal extraction and gel preparation conditions of salt-soluble proteins from S. zygaena muscle were 0.90mol/L NaCl, pH7.0 and 35min of low-temperature heating time. Experimentally, these conditions produced a salt-soluble protein gel with a WHC of 72%, confirming the model prediction.}

Key words: Sphyrna zygaena, Salt-soluble protein, Water holding capacity (WHC) of gel, Respond surface methodology