引用本文:	徐梅英,李继姬,郭宝英,吕振明,周超,吴常文.中国沿海 7 个长蛸(Octopus variabilis)野生群体的遗传多样性.海洋与湖沼,2011,42(3):387-396.

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*中国沿海 7 个长蛸(Octopus variabilis)野生群体的遗传多样性*
徐梅英^1,2, 李继姬¹, 郭宝英¹, 吕振明¹, 周超¹, 吴常文¹
1.浙江海洋学院海洋科学院浙江省海洋养殖装备与工程技术重点实验室;2.浙江大海洋科技有限公司

摘要:

基于线粒体 DNA 的 12S rRNA 和 COⅢ基因序列对我国沿海重要经济头足类长蛸不同群体(大连、烟台、青岛、连云港、舟山、温州、厦门)进行了遗传多样性和遗传结构的分析。由 PCR 扩增获得 80 个个体的 12S rRNA 基因 416bp、COⅢ基因 512bp 的部分序列, 两者多态性遗传参数统计显示, 80个个体分别检出 64 (12S)和 27 (COⅢ)个单倍型, 总群体单倍型多样性指数(Hd)分别为 0.984 (12S)和 0.909 (COⅢ), 核苷酸多样性指

关键词: 长蛸, 12S rRNA, COⅢ, 遗传多样性, 分子标记技术

DOI：10.11693/hyhz201103008008

分类号:

基金项目:国家高技术研究发展计划(863 计划)项目“名贵头足类苗种规模繁育关键技术”, 2010AA10A404 号; 国家海洋公益性行业科研专项, 201005013 号; 海洋渔业科学与技术浙江省重中之重学科开放课题, 20100105 号; 浙江海洋学院人才引进启动项目, 21135011509 号

附件

GENETIC DIVERSITY OF SEVEN POPULATIONS OF OCTOPUS VARIABILIS IN CHINA’S COASTAL WATERS BASED ON THE 12S rRNA AND COⅢ GENE ANALYSIS

XU Mei-Ying^1,2, LI Ji-Ji¹, GUO Bao-Ying¹, Lü Zhen-Ming¹, ZHOU Chao¹, WU Chang-Wen¹

1.Marine Science College of Zhejiang Ocean University, Key Laboratory of Mariculture Equipments and Engineering Technology of Zhejiang Province;2.Zhejiang Dahaiyang Science and Technology Co., Ltd.

Abstract:

In this study, the genetic diversity and genetic structure of seven wild populations of Octopus variabilis in China’s coastal waters (Dalian, Yantai, Qingdao, Lianyungang, Zhoushan, Wenzhou and Xiamen) were analyzed using the mitochondrial gene sequencing technology (12S rRNA and COⅢ). The result revealed 64 haplotypes using the 12S rRNA gene and 27 haplotypes using the COⅢ gene for total 80 individuals obtained using PCR method. The haplotype diversities (Hd) were 0.9840 (12S) and 0.9092 (COⅢ), respectively; the nucleotide diversities (Pi) were 0.0277 (12S) and 0.0337 (COⅢ), respectively; and the average nucleotide differences (K) were 9.403 (12S) and 17.265 (COⅢ), respectively, showing high genetic diversity among populations. The genetic distances between the Xiamen population and other populations were up to 0.072 (12S) and 0.102 (COⅢ), respectively, showing considerable genetic differentiation, which is probably caused by different hydrological conditions in the Xiamen site than other sites.

Key words: Octopus variabilis, 12S rRNA, COⅢ, Genetic diversity, Molecule marker technology