引用本文:	陈群芳,汤文仲,冯子慧,霍元子,何培民.长石莼(缘管浒苔)(Ulva linza)原生质体再生与分化发育初步研究.海洋与湖沼,2011,42(3):397-403.

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*长石莼(缘管浒苔)(Ulva linza)原生质体再生与分化发育初步研究*
陈群芳^1,2, 汤文仲^1,2, 冯子慧^1,2, 霍元子^1,2, 何培民^1,2
1.上海海洋大学水产与生命学院;2.上海海洋大学海洋科学研究院

摘要:

采用细胞酶解技术获得长石莼(缘管浒苔)原生质体, 再通过细胞培养观察原生质体再生以及各种分化发育途径。结果表明, 长石莼(缘管浒苔)主要存在 3 种分化发育途径: 1) 部分原生质体可直接分裂形成有假根或无假根得单细胞苗。2) 部分原生质体分裂成规则细胞团或不规则细胞团, 最后形成苗簇。3) 部分原生质体可发育为孢子囊/配子囊, 其中部分孢子囊/配子囊成熟后释放出游孢子/配子, 可直接形成小苗。雌雄配子以正面结合(大部分)也可以首尾交错结合(少部分)形成合子并萌发形成小苗。研究表明,

关键词: 长石莼(缘管浒苔), 细胞酶解分离, 原生质体培养, 细胞再生, 分化发育途径

DOI：10.11693/hyhz201103009009

分类号:

基金项目:国家海洋局绿潮灾害专项, LZ-03-01 号; 国家海洋局公益性行业科研专项, 2012418010 号; 海洋赤潮灾害立体监测技术与应用国家海洋局重点实验室开放研究基金项目, MATHAB200912 号; 国家自然科学基金项目, 30371101 号; 教育部博士点基金资助项目, 2007—2008; 上海市浦江人才计划项目, 05PJ14086 号; 上海市水生生物学重点学科资助项目, S30701 号; 上海高校选拔培养优秀青年教师科研专项基金, SSC08002 号。

附件

PILOT STUDIES OF REGENERATION AND DEVELOPMENT OF PROTOPLASTS FROM ULVA LINZA

CHEN Qun-Fang^1,2, TANG Wen-Zhong^1,2, FENG Zi-Hui^1,2, HUO Yuan-Zi^1,2, HE Pei-Min^1,2

1.College of Aqua-life Science and Technology, Shanghai Ocean University;2.Institute of Marine Science, Shanghai Ocean University

Abstract:

In this paper, cell regeneration, differentiation and development of Ulva linza protoplasts were studied. Individual cells were enzymatically isolated from Ulva linza blades, and the cells and protoplasts were cultured and regenerated into young plants. The results showed there were three main differentiations and development pathways for isolated cells: 1) Partial protoplasts directly divided and formed the single cell seedlings with or without a rhizoid; 2) Partial protoplast divided into cell aggregates including irregular and regular cell aggregates; both could form seedlings directly. 3) Partial protoplasts divided and developed into zoosporangia or gametangia. Zoosporangia could release zoospores and then germinated into young sporelings. Gametangia could release gametes, and male and female gametes conjugated with head-tail style or tail-tail style into one zygote, which could germinate into seedling. Usually, one somatic cell in blade could form into one single-cell seedling or 8—32 sporelings through zoosporangium or gametangium development way. It was found that the cells in Ulva linza had a great reproductive capacity. This study has laid a foundation for further research on green tide blooming and the life history of Ulva linza.

Key words: Ulva linza, Isolated cells by enzymes, Cell culture, Cell regeneration, Differentiation and development