| 摘要: |
| 采用COⅠ基因片段研究了东海区象山(XS)、乐清(YQ)和宁德(ND)三个四指马鲅群体的遗传多样性和种群遗传结构。在90个四指马鲅个体中, 共检出单倍型13个, 包括变异位点23个, 其中简约信息位点14个。在三个种群中, YQ 群体具有最高的单倍型多样性和核苷酸多样性水平, 但整体上, 三个群体的遗传变异水平均比较低。中性检验结果表明, 三个群体内部在分子水平可能存在自然选择作用。NJ系统发生分析发现, YQ 部分单倍型与XS和ND群体聚在一起, 而与另一部分YQ群体遗传分化较大。AMOVA分析显示, 遗传差异主要来自于群体内部(79.66%)。 |
| 关键词: 四指马鲅, 细胞色素c氧化酶亚基Ⅰ(COⅠ), 种群结构, 遗传多样性 |
| DOI：10.11693/hyhz201206033033 |
| 分类号: |
| 基金项目:浙江省科技厅创新团队建设与人才培养项目, 2011F20033 号; 温州市重点科技创新团队项目, C20120004 号; 国家海洋局“海洋公益性行业科研专项”, 201105009-3 号。 |
附件 |
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| POPULATION GENETIC STRUCTURE OF ELEUTHERONEMA TETRADACTYLUM(SHAW) IN EAST CHINA SEA BASED ON SEQUENCE ANALYSIS OF COⅠGENE |
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LIN Shao-Zhen1, WANG Dan-Li2, WANG Ya-Jun2, YAN Xiao-Jun2
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1.Zhejiang Mariculture Research Institute;2.School of Marine Sciences, Ningbo University
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| Abstract: |
| Population genetic structure and genetic diversity of Eleutheronema tetradactylum(Shaw) in East China Sea were evaluated by mitochondrial COⅠpartial sequences. A total of 90 individuals were analyzed, and 23 polymorphic sites (including 14 parsimony informative sites) defined 13 distinct haplotypes. Yueqing (YQ) population had the highest values of both haplotype diversity (h) and nucleotide diversity (π), but the levels of h and π were generally low in all three populations. Neutral evolution test and mismatch distribution indicated historical demographic expansion happened in three populations. NJ phylogenetic tree indicated that haplotypes from XS (Xiangshan population), ND (Ningde population) and part of YQ population pooled together, which had high genetic divergence from other haplotypes in YQ population. Analysis of molecular variance (AMOVA) indicated that variations were mainly within population (79.66%). The present results provide new information for genetic assessment, fishery management and conservation of this species. |
| Key words: Eleutheronema tetradactylum(Shaw), Cytochrome coxidase subunitⅠ(COⅠ), Population structure, Genetic diversity |
