引用本文:	孔蕾,朱凝瑜,贝亦江,丁雪燕,陈健舜.中华鳖(Pelodiscus sinensis)摩氏摩根菌(Morganella morganii)的鉴定及致病性研究.海洋与湖沼,2013,44(3):722-727.

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*中华鳖(Pelodiscus sinensis)摩氏摩根菌(Morganella morganii)的鉴定及致病性研究*
孔蕾^1,2, 朱凝瑜², 贝亦江², 丁雪燕², 陈健舜²
1.浙江大学动物预防医学研究所;2.浙江省水产技术推广总站

摘要:

从3只患有头面部疖疮中华鳖(Pelodiscus sinensis)的肝脏、肾脏、头面部病灶干酪样组织, 分离得到12株细菌。综合菌落形态观察、16S rRNA序列分析与生化特性分析方法, 所有分离株均为摩氏摩根菌(Morganella morganii)。稚鳖和小鼠感染试验提示该菌具有较强的致病力, 其对小鼠的半数致死量(LD₅₀)为10^7.24。同时采用常规琼脂扩散(K-B)法进行8类14种常用药物的抗菌敏感性测定, 结果表明, 分离株对氨基糖苷类、?-内酰胺类、四环素类、林可酰胺类、多肽类、喹诺酮类、磺胺类等中的8种药物耐药, 对氨曲南、氟苯尼考、头孢哌酮则较为敏感。本研究为中华鳖以头面部疖疮为主要临床特征的摩氏摩根菌病的首例报道, 旨在为该病的确诊与防治提供科学依据和参考。

关键词: 中华鳖摩氏摩根菌鉴定致病力耐药性

DOI：10.11693/hyhz201303028028

分类号:

基金项目:浙江省重大科技专项, 2012C12907-1 号; 浙江省水产技术推广总站站长基金资助项目, TGZ201101 号

附件

ISOLATION AND IDENTIFICATION OF PATHOGENIC MORGANELLA MORGANII ISOLATES FROM CHINESE SOFT-SHELLED TURTLE PELODISCUS SINENSIS

KONG Lei^1,2, ZHU Ning-Yu², BEI Yi-Jiang², DING Xue-Yan², CHEN Jian-Shun²

1.Zhejiang University, Institute of Preventive Veterinary Medicine;2.Zhejiang Fisheries Technical Extension Center

Abstract:

An outbreak of Chinese soft-shelled turtle (Pelodiscus sinensis) disease with typical symptoms of head and facial furuncle sore happened in a large-scale breeding farm in Zhejiang Province, 2012. A total of 12 isolates were collected from livers, kidneys and facial loci of these diseased turtles. Based on the colony morphology, biochemical reactions and the sequence analysis of 16S rRNA gene, these isolates were identified as Morganella morganii. These isolates demonstrated virulence potential both in Chinese soft-shelled turtle and mouse models, with the median lethal dose (LD₅₀) in mice at 10^7.24. The susceptibity test to antibiotics using Kirby-Bauer Sagar diffusion method showed these M. morganii isolates were resistant to aminoglycosides, beta-lactams, tetracyclines, lincomycin amides, polypeptides, quinolones, sul-fonamides, and sensitive to aztreonam, florfenicol, cefoperazone. This study represents the first report of M. morganii-mediated disease with the typical signs of head and facial furuncle sore in Chinese soft-shelled turtles, and paves the way for the prevention and treatment program of this disease.

Key words: Chinese soft-shelled turtle Pelodiscus sinensis Morganella morganii identification pathogenicity antimicrobial sensitivity