| 摘要: |
| 为评估三疣梭子蟹(Portunus trituberculatus)体重及其低盐耐受性遗传力,本实验构建了20个三疣梭子蟹全同胞家系,分别在其60、90养殖日龄测量其各个家系的体重,并在60日龄时取每个家系90个个体,在盐度4胁迫下分别在12h、24h、48h、72h统计其死亡率,作为衡量其盐度耐受性指标。利用全同胞方差组分分析法评估其低盐耐受性及体重遗传参数。结果发现三疣梭子蟹在60日龄下,其体重遗传力为0.45,其在12h、24h、48h、72h处理条件低盐耐受性遗传力分别为0.13、0.18、0.21、0.29。通过聚类分析的方法将其耐受性状分为3类,并通过相关性分析发现三疣梭子蟹生长性状与低盐耐受性性状的相关系数为0.143,检验并不显著(P=0.547),说明三疣梭子蟹生长性状与耐低盐性状并不相关,在选育时可采用复合选育,提高育种效率。 |
| 关键词: 三疣梭子蟹 体重 低盐耐受性 遗传参数 |
| DOI：10.11693/hyhz20150500134 |
| 分类号: |
| 基金项目:国家自然科学基金项目,41476124号;国家863项目,2012AA10A409号;浙江省水产育种专项,2012C12907-3号;浙江省公益性技术应用研究项目,2013C31032号;宁波市海洋蟹类产业科技创新团队,2011B81003号。 |
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| ESTIMATION ON GENETIC PARAMETERS FOR LOW SALINITY TOLERANCE AND BODY WEIGHT OF PORTUNNUS TRITUBERCULATUS |
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LU Shao-Kun1, LI Rong-Hua1, MU Chang-Kao1, SONG Wei-Wei1, ZHENG Wei-Bing2, WANG Chun-Lin1
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1.School of Marine Sciences, Ningbo University, Ningbo 315211, China;2.Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
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| Abstract: |
| We built up 20 full-sib families of Portunus trituberculatus to evaluate its genetic parameters of body weight and low-salinity tolerance. All the families were cultured under same conditions. After 60 and 90 days, body weight was measured and 90 individuals that randomly selected from each of the 20 families were placed under salinity 4, and mortality was counted in 12, 24, 48, and 72h. The mortality was calculated to indicate the low-salinity tolerance. In addition, we conducted full-sibs variance component analysis to evaluate the genetic heritability of body weight. Results show that the heritability of body weight in 60-day-old animals was 0.45, and that of low-salinity tolerance in 12, 24, 48, and 72h was 0.13, 0.18, 0.21, and 0.29, respectively. Moreover, we divided the low-salinity tolerance into 3 levels by K-means clustering. No significant correlation was found between the body weight and the low-salinity tolerance. Therefore, hybrid selective breeding technology was suggested for genetic improvement in production and tolerance traits in the future. |
| Key words: Portunus trituberculatus body weight low-salinity tolerance genetic parameters |
