摘要: |
在人工条件下采用静水封闭呼吸瓶的方法研究了温度、盐度对泥蚶(Tegillarca granosa)呼 吸、排泄的影响, 并综合分析了温度、盐度交互作用对呼吸、排泄的影响程度, 为泥蚶基础生物学研 究积累了资料。结果表明, 在设定的不同温度条件下泥蚶耗氧率变化范围0.63-4.47mg/(g·h), 单因 素方差分析结果显示4 个盐度条件下泥蚶耗氧率的差异均极显著(P<0.01); 排氨率的变化范围 123.11-955.68μg/(g·h), 排氨率受温度的影响也均极显著(P<0.01); 经回归发现, 在温度13-31℃ 范 围内, 耗氧率和排氨率均与温度的变化呈显著的指数相关。除温度为18℃ 时, 其它各温度条件下, 不同盐度梯度间的耗氧率差异不显著(P>0.05), 盐度对排氨率影响的变化趋势不一致。在温度 (13-31℃)和盐度(18-28)时, 温度和盐度对耗氧率、排氨率的影响均存在交互作用, 耗氧率(Y)、排 氨率(Y)与温度(T)和盐度(S)之间的二元线性回归关系为Y=a+bT+cS+dT×S.泥蚶呼吸排泄的O:N 比 在4.50-13.07 之间, 平均值为7.16.泥蚶呼吸排泄的代谢活动受温度、盐度的影响显著。 |
关键词: 泥蚶 温度 盐度 呼吸 排泄 |
DOI:10.11693/hyhz20141200347 |
分类号: |
基金项目:公益性行业(农业)科研专项项目, 201303047 号; 宁波市科技计划重大项目, 2014C10018 号; 欧盟第六框架项目, INCOCT- 2004-510706 号。 |
附件 |
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EFFECTS OF TEMPERATURE AND SALINITY ON OXYGEN CONSUMPTION AND AMMONIA EXCRETION OF MUDDY SHELLFISH TEGILLARCA GRANOSA |
JIAO Hai-Feng1,2, YAN Qiao-Na3, ZHENG Dan1,2, YOU Zhong-Jie1,2, YAN Xiao-Jun2
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1.Ningbo Academy of Oceanology and Fishery, Ningbo 315012, China;2.Ocean College of Ningbo University, Ningbo 315211, China;3.Jiangbei Ocean and Fishery Bureau, Ningbo 315020, China
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Abstract: |
We determined the effects of water temperature and salinity on oxygen consumption and ammonia excretion of Tegillarca granosa in an experiment in still water and sealed respiratory bottle. T. granosa was exposed to six temperatures (13, 18, 23, 28, 31, 34℃) and four salinities (18, 23, 28, 33). The results show that the oxygen consumption rate varied from 0.63 to 4.47mg/(g·h) and the ammonia excretion rate from 123.11 to 955.68μg/(g·h) against the temperatures. Both oxygen consumption rate and ammonia excretion rate were correlated significantly to temperature variation (P<0.01) and could be described by logarithmic equations. To salinity, however, the ammonia excretion changed irregularly, while significantly with oxygen consumption at 18℃ only. The interactions of oxygen consumption and ammonia excretion against water temperature and salinity in our designed ranges were found obvious. The ratio of O: N varied from 4.50 to 13.07, under which normal metabolism could be maintained in T. granosa. |
Key words: Tegillarca granosa water temperature salinity oxygen consumption ammonia excretion |