首页 | 期刊简介 | 编委会 | 投稿指南 | 常用下载 | 联系我们 | 期刊订阅 | In English
引用本文:伍琴,唐建洲,刘臻,赵玉蓉,郝光,鲁双庆.牛磺酸对鲫鱼(Carassius auratus)生长、肠道细胞增殖及蛋白消化吸收相关基因表达的影响.海洋与湖沼,2015,46(6):1516-1523.
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 2065次   下载 1844 本文二维码信息
码上扫一扫!
分享到: 微信 更多
牛磺酸对鲫鱼(Carassius auratus)生长、肠道细胞增殖及蛋白消化吸收相关基因表达的影响
伍琴1,2, 唐建洲2, 刘臻3,2, 赵玉蓉1, 郝光2, 鲁双庆2
1.湖南农业大学动物科技学院 长沙 410128;2.长沙学院生物与环境工程系 长沙 410003;3.湖南省水产高效健康生产协同创新中心 常德 415000
摘要:
本文探讨牛磺酸在鲫鱼生长、肠道细胞增殖分化以及蛋白质消化吸收相关基因的表达等方 面的作用。试验以480 尾[初始体质量(29.07±0.19)g]鲫鱼(Carassius auratus)为研究对象, 随机分成6 组, 每组设定4 个重复, 每个重复有鱼20 尾。鲫鱼基础饲料中添加的牛磺酸的剂量分别是0.0、1.0、 2.5、4.0、6.0 以及9.0 g/kg, 以0.0 g/kg 添加组为对照组, 试验持续8 周。结果表明: (1) 牛磺酸对鲫 鱼有促生长作用, 6.0 g/kg 添加组的鱼的增重率, 特定生长率, 蛋白质效率, 饲料系数等都显著高于 对照组(P<0.05).(2) 牛磺酸能促进肠绒毛的生长, 降低隐窝深度。(3) 牛磺酸提高了鲫鱼血清中T3、 T4 的含量, 添加6.0 g/kg 时显著高于对照组, 且此时血清尿素氮浓度显著低于对照组和其它处理组 (P<0.05).(4) 氨肽酶N(APN)、小肽转运蛋白(PepT1)、基因系尾型同源盒基因(CDX2)、L-氨基酸转 运载体蛋白(LAT2)等基因的相对表达量随牛磺酸剂量增加而上调, 6.0 g/kg 时显著高于对照组和其它 实验组(P<0.05).由此可见, 适量添加牛磺酸能影响鲫鱼生长、肠道细胞增殖及蛋白消化吸收相关基 因的表达。
关键词:  牛磺酸  鲫鱼  生长  蛋白质  细胞增殖
DOI:10.11693/hyhz20150800227
分类号:
基金项目:国家自然科学基金项目, 31272675 号; 湖南省教育厅优秀青年项目, 13B144 号; 湖南省高校产业化培育项目, 13CY031 号。
附件
PROPER TAURINE-ENRICHED FISHMEAL PROMOTES GROWTH OF CRUCIAN CARP CARASSIUS AURATUS
WU Qin1,2, TANG Jian-Zhou2, LIU Zhen3,2, ZHAO Yu-Rong1, HAO Guang2, LU Shuang-Qing2
1.College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;2.Department of Biotechnology & Environment Engineering, Changsha University, Changsha 410003, China;3.Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China
Abstract:
To study the effect of taurine-enriched feed on growth of Carassius auratus, taurine in different concentrations (0.0, 1.0, 2.5, 4.0, 6.0, and 9.0 g/kg) was tested in 8 weeks, during which proliferation of intestinal cell and expressions of genes associated with protein digestion and absorption were studied. A total of 480 individuals in body weight of 29.07±0.19g were selected and randomly divided into 6 treatments containing 4 replicates each with 20 individuals. The basal diet with no taurine was used as the control. The results show that taurine could promote the growth performance best at 6.0 g/kg, at which the weight gain rate, specific growth rate, feed coefficient, and protein efficiency ratio increased remarkably than the control (P>0.05). In specific, taurine-addition promoted the growth of intestinal villus, reduced crypt depth remarkably, enhanced the serum T3 and T4 concentrations, and reduced serum urea nitrogen concentration significantly (P>0.05). In addition, taurine enhanced remarkably the relative expression of APN, PepT1, LAT2, and CDX2 mRNA. Therefore, a proper amount of taurine supplementation is necessary for the proliferation of intestinal cell, genes associated with protein digestion and absorption of the fish, favoring the growth performance.
Key words:  taurine  crucian carp  growth  protein  cellular proliferation
版权所有 海洋与湖沼 Oceanologia et Limnlolgia Sinica Copyright©2008 All Rights Reserved
主管单位:中国科协技术协会 主办单位:中国海洋湖沼学会
地址:青岛市海军路88号  邮编:266400  电话:0532-82898753  E-mail:ols@qdio.ac.cn
技术支持:北京勤云科技发展有限公司