| 引用本文: | 舒琥,岳磊,李强,蓝昭军,谷平华,王杭军,吴土金.不同流域光倒刺鲃(Spinibarbus hollandi)子一代及其亲本的线粒体COI 基因遗传变异分析.海洋与湖沼,2015,46(6):1509-1515. |
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| 摘要: |
| 对2 组光倒刺鲃(Spinibarbus hollandi)杂交子一代(长江♀×北江♂子一代, 北江♀×长江♂子 一代)及其亲本(北江♀、♂, 长江♀、♂)的线粒体COI 基因序列进行了分析。在18 个样品中共检测到 8 个单倍型和35 个核苷酸多态位点。通过序列差异分析和遗传距离比较发现, 核苷酸序列同源性在 98.1%-99.9%之间, 遗传分化不明显。杂交子一代的5 个单倍型与其母本的2 个单倍型的核苷酸同 源性在99.4%-99.8%之间。而与父本的同源性分别为98.5%、98.2%、98.2%、98.4%、98.1%.实验 结果表明两种杂交子一代的线粒体 COI 基因严格遵循母性遗传规律。 |
| 关键词: 光倒刺鲃 杂交子代 COI 遗传变异 |
| DOI：10.11693/hyhz20150800229 |
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| 基金项目:国家公益性行业(农业)重大专项, 201303048; 广东省科技计划项目, 2012A020602063 号, 2014A020208145 号。 |
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| GENETIC VARIABILITY OF mtDNA COI GENES IN SPINIBARBUS HOLLANDI |
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SHU Hu1, YUE Lei1, LI Qiang1, LAN Zhao-Jun2, GU Ping-Hua2, WANG Hang-Jun2, WU Tu-Jin2
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1.School of Life Science, Guangzhou University, Guangzhou 510006, China;2.Shaoguan Fisheries Research Institute, Shaoguan 512006, China
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| Abstract: |
| To identify phylogenetic variability in Spinibarbus hollandi hybrids from two populations, their mitochondrial cytochrome oxidase subunit I (COI) gene fragments were amplified in PCR (Polymerase Chain Reaction), of which a 1551-bp base-pair nucleotide sequence was examined. Eighteen samples were divided into 6 groups, from which 8 haplotypes and 35 nucleotide polymorphic loci COI gene fragments were recognized and they shared high similarities from 98.1% to 99.9% in nucleotide sequence revealed by sequence divergence and genetic distance analyses, indicating no significant genetic difference among these groups. In five COI haplotypes of the first-generation hybrids, the similarity was 99.4%-99.8% to the female parents and 98.1%-98.5% to the male ones. Therefore, the hybrids inherited more from their maternal parent in mitochondrial COI genes, following strictly the rules of maternal inheritance. |
| Key words: Spinibarbus hollandi hybrids COI genetic variability |
