| 摘要: |
| 大叶藻海草床的退化和恢复受到国内外学者的广泛关注，而大叶藻种子萌发则是恢复实践中的一项关键技术。为了提高大叶藻种子萌发率，本研究在实验室可控条件下，采用正交实验对影响大叶藻种子萌发的环境因素（盐度、温度、N/P营养盐浓度及pH）进行了优化，并在优化条件下进一步探究了光质类型、UV-B辐射、低温层积和划破种皮对大叶藻种子萌发和平均萌发时间的影响。结果表明：盐度12、温度10°C、N/P营养盐浓度960/60μmol/L、pH 6.2是大叶藻种子相对适宜的萌发条件组合；黑暗条件下大叶藻种子萌发率最高，为92%，不同类型光质照射不促进大叶藻种子萌发率的提高，但红光照射可在短期内加速种子萌发；在一定强度范围内，种子萌发率随UV-B辐射时长的增加而升高，每日辐射8h，处理8周后种子萌发率可达93%。划破种皮和低温层积均可显著提高大叶藻种子的萌发率并缩短平均萌发时间，经划破种皮处理后，2周内种子萌发率可提高至91%。间 |
| 关键词: 正交实验 大叶藻种子 萌发率 平均萌发时间 |
| DOI：10.11693/hyhz20151100285 |
| 分类号: |
| 基金项目:海洋公益性行业科研专项经费资助项目，201305009号；国家自然科学基金项目，41476091号；青岛市博士后人员应用研究项目，861605040064号。 |
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| OPTIMAL CONDITION FOR SEED GERMINATION OF ZOSTERA MARINA |
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WANG Ming1, GUO Xin2, TANG Xue-Xi1, ZANG Yu1, YAN Wen-Jie1, ZHOU Bin1
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1.Collage of Marine Life Science, Ocean University of China, Qingdao 266003, China;2.Collage of Environment and Ecology, Xiamen University, Xiamen 361102, China
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| Abstract: |
| In recent years, vegetation of seagrass species Zostera marina has been degenerated in many places around world. To solve this problem, seed germination is the key for the seagrass bed restoration. To examine the optimal combination effect of four single environmental factors (salinity, temperature, N/P concentration, and pH) on the seed germination, we conducted an orthogonal experiment under controlled indoor conditions. The results show that the optimal condition was salinity 12, 10°C, N/P concentration 960/60μmol/L, and pH 6.2. On top of that, we investigated the influences of ultraviolet B radiation, lighting (different in color, duration, and intensity), cold stratification, and seed coat scarification on the germination, and found that dark condition (the germination rate reached 92%) but lighting properties was favorable, except for short red illumination treatment which enhanced the germination somewhat. In addition, ultraviolet B radiation boosted the germination significantly (P<0.05) within a certain period of radiation. Both cold stratification and seed coat scarification could improve the germination and shorten the germination time significantly (P<0.05). The experimental results provide a theoretical basis for the indoor seeding cultivation of Z.marina, and may help the success of application to restore the ecosystem of seagrass bed. |
| Key words: Orthogonal experiment Zostera marina seed germination rate mean germination time |
