引用本文:	邓益琴,赵晶晶,刘松林,赵哲,陈偿.Hfq对溶藻弧菌(Vibrio alginolyticus)毒力的调控分析.海洋与湖沼,2016,47(3):604-611.

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*Hfq对溶藻弧菌(Vibrio alginolyticus)毒力的调控分析*
邓益琴^1,2,3, 赵晶晶^1,2, 刘松林^1,3, 赵哲^1,2, 陈偿^1,2,4
1.中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室广州 510301;2.中国科学院南海海洋研究所广东省应用海洋生物学重点实验室广州 510301;3.中国科学院大学北京 100049;4.中国科学院南海海洋研究所西沙、南沙深海海洋环境观测研究站广州 510301

摘要:

为探讨分子伴侣Hfq对溶藻弧菌（Vibrio alginolyticus）毒力的调控作用，本文分析了溶藻弧菌ZJ-T野生株、hfq突变株及回补株各项与毒力相关的生理生化指标。研究结果显示：在半固体和固体LBS平板中，缺失株游动和涌动能力显著降低（P<0.001）；hfq缺失株生物膜合成速度及合成量降低，但解离速度增加；在限铁环境下，缺失株生长有所减弱；缺失株胞外蛋白酶分泌增强（P<0.01）；同野生株和突变株相比，缺失株对小鼠成肌细胞系C2C12细胞几乎不致死，且其对石斑鱼的半数致死量提高3个数量级。实验结果表明：Hfq对溶藻弧菌毒力具有重要调控作用，并通过调控其运动、生物膜形成、铁代谢、胞外蛋白酶分泌等生理生化过程从而调控其毒力。本研究可为溶藻弧菌病害暴发的防治提供重要理论依据。

关键词: 溶藻弧菌 Hfq 毒力

DOI：10.11693/hyhz20160100022

分类号:

基金项目:国家自然科学基金项目，31272697号，41276163号；广州市珠江科技新星项目，2013J2200094号；广东省省级科技计划项目，2014B030301064号。

附件

ROLE OF Hfq IN VIRULENCE REGULATION OF VIBRIO ALGINOLYTICUS

DENG Yi-Qin^1,2,3, ZHAO Jing-Jing^1,2, LIU Song-Lin^1,3, ZHAO Zhe^1,2, CHEN Chang^1,2,4

1.Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;2.Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;3.University of Chinese Academy of Sciences, Beijing 100049, China;4.Xisha/Nansha Ocean Oberservation and Research Station, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China

Abstract:

To understand the role of Hfq in the virulence of Vibrio alginolyticus, we studied a series of virulence-related physiological and biochemical pathways comparatively among V. alginolyticus wild type strain ZJ-T, hfq mutant strain Δhfq-T, and the complementary strain hfq⁺-T. The results show that the swimming and swarming abilities of hfq null strain were significantly reduced (P<0.001). Deletion of hfq decreased the biofilm formation while promoted the deattachment of V. alginolyticus. Hfq positively regulated iron metabolism and negatively regulated the secretion of extracellular proteases (ECPs) (P<0.01). Compared to the wild type and complementary strains, the hfq deficient strain completely lost the ability for inducing C2C12 cell death, and increased lethal dose (LD₅₀) to Epinephelus awoara by three orders of magnitude. Therefore, Hfq is crucial in the virulence of V. alginolyticus by regulating motility, bofilm formation, iron metabolism, and secretion of ECPs. This study may provide an insightful theoretical basis for the prevention of vibriosis.

Key words: Vibrio alginolyticus Hfq virulence