引用本文:	叶键,刘晓宁,王晓,徐慧,沈文英,高敏杰,章晓栋.青鱼(Mylopharyngodon piceus)新发病病原类志贺邻单胞菌(Plesiomonas shigelloides)的分离鉴定.海洋与湖沼,2016,47(3):633-639.

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*青鱼(Mylopharyngodon piceus)新发病病原类志贺邻单胞菌(Plesiomonas shigelloides)的分离鉴定*
叶键¹, 刘晓宁², 王晓³, 徐慧³, 沈文英⁴, 高敏杰⁴, 章晓栋⁴
1.杭州市水产技术推广总站杭州 310001;2.杭州大江东产业集聚区农业局杭州 311222;3.绍兴出入境检验检疫局绍兴 312000;4.绍兴文理学院生命科学学院绍兴 312000

摘要:

为研究青鱼暴发性死亡原因，从病症典型青鱼（Mylopharyngodon piceus）肌肉、肝脏和肾脏处进行细菌接种，分离获得一株细菌（HZ2015），对其进行了形态观察、生化特性检测和16S rRNA序列分析，通过动物回归实验、毒力检测和药敏试验了解其对青鱼的致病性、毒力及药物敏感性。所得菌株为类志贺邻单胞菌（Plesiomonas shigelloides），动物回归实验能复制出类似症状，且能从体内再次分离到该菌株；分离株对小鼠的半数致死量（LD₅₀）为10^6.6 CFU；对头孢呋辛、头孢哌酮、庆大霉素、卡那霉素、复达欣高度敏感。研究表明，菌株HZ2015为青鱼体表溃烂病病原，本研究系类志贺邻单胞菌致青鱼体表溃烂病的首次报道。

关键词: 青鱼类志贺邻单胞菌强致病性

DOI：10.11693/hyhz20160300049

分类号:

基金项目:国家自然基金青年科学基金资助项目，31201930号；浙江省公益技术研究农业项目，2013C32045号；绍兴市公益技术应用研究项目2014B70044号。

附件

ISOLATION AND IDENTIFICATION OF PATHOGENIC PLESIOMONAS SHIGELLOIDES FEOM DISEASED BLACK CARP MYLOPHARYNGODON PICEUS

YE Jian¹, LIU Xiao-Ning², WAN Xiao³, XU Hui³, SHEN Wen-Ying⁴, GAO Min-Jie⁴, ZHANG Xiao-Dong⁴

1.Hangzhou Aquacultural Techica Extending Centre, Hangzhou 310001, China;2.Hangzhou Dajiangdong Industrial District Agricultural Bureau, Hangzhou 311222, China;3.Shaoxing Entry-exit Inspection and Quaratine Bureau, Shaoxing 312000, China;4.College of Life Sciences, Shaoxing University, Shaoxing 312000, China

Abstract:

In a recent outbreak of massive death of black carp Mylopharyngodon piceus in a breeding farm in Hangzhou, showing ulcerated body surface, we isolated a bacterium from liver, kidney, and muscle of diseased fish. In morphology, Gram’s staining, ATB ID32E bacterial biochemical identification, and sequence analysis on the 16S rRNA gene, the isolate was identified as Plesiomonas shigelloides, named HZ2015. In our regress experiment, similar symptoms replayed. The same bacterium could be identified biochemically in 16S rDNA homology analysis. Isolate HZ2015 was demonstrated with virulence potential in mouse model, and the median lethal dose (LD₅₀) in mice was 10^6.6 CFU/mL. Therefore, we deduce that P. shigelloides was the pathogenic bacteria responsible for the disease. In addition, in our susceptibility test on antibiotics, the bacterial strain HZ2015 was susceptible to cefoperazone, cefuroxime, gentamicin, kanamycin, and ceftazidime. This study presents the first case report on P. shigelloides-induced massive death of black carp M. piceus in China.

Key words: Mylopharyngodon piceus Plesiomonas shigelloides strong pathogenicity