| 摘要: |
| 从发病死亡的养殖太平洋双色鳗鲡（Anguilla bicolor pacifica）肝脏中分离到一株优势菌AMSH1，为研究其种属、毒力及中药敏感性，结合BIOLOG微生物鉴定系统和16S rDNA序列分析对该菌株进行鉴定，并通过人工回归感染试验确定其致病性。此外，还研究了10味中药单用、10种双联用和4种三联用对该菌株的体外抑菌作用。结果显示该菌株为类志贺邻单胞菌（Plesiomonas shigelloides）。经人工腹腔注射感染健康太平洋双色鳗鲡，证实该菌具有致病性，半致死量LD50为3.25;105CFU/g。药敏试验结果表明，10味中药均有较好的抑菌效果，其中五倍子（Galla chinensis）、丁香（Eugenia caryophllata）和生地榆（Sanguisorba officinalis）的效果最好，7种双联用复方和1种三联用复方均具有协同抑菌作用。本次引起太平洋双色鳗鲡发病的病原为类志贺邻单胞菌，该菌对太平洋双色鳗鲡的致病性及其中药药敏的研究属首次报道。建议选用含五倍子的中药双联用复方或三联用复方进行防治，皆绿色环保。 |
| 关键词: 太平洋双色鳗鲡 类志贺邻单胞菌 病原菌 药敏试验 |
| DOI：10.11693/hyhz20160400090 |
| 分类号: |
| 基金项目:福建省自然科学基金项目，2013J05052号；福建省科技厅区域发展项目，2016N3002号；厦门市科技局项目，3502Z20133015号；鳗鲡现代产业技术教育部工程研究中心开放基金，RE201308号；福建省教育厅项目，JA15260号。 |
附件 |
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| PATHOGENIC PLESIOMONAS SHIGELLOIDES ISOLATED FROM ANGUILLA BICOLOR PACIFICA: IDENTIFICATION AND SENSITIVITY TO CHINESE HERBS |
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LAI Xiao-Jian1,2, YANG Fang-Yuan1,2, LI Zhong-Qin1,2
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1.Fisheries College, Jimei University, Xiamen 361021, China;2.Engineering Research Center of the Modern Industrial Technology for Eel, Ministry of Education, Xiamen 361021, China
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| Abstract: |
| In May,2012,a massive mortality of Anguilla bicolor pacifica broke out in an aquaculture farm in Fuqing,Fujian Province.Symptoms included congestive and anabrotic intestinal mucosa,and oncotic liver.We isolated pathogenic bacterium,named AMSH1,from the liver of diseased A.bicolor pacifica,on which the classification,virulence,and sensitivity of the bacterium to Chinese herbs were studied.The strain was identified by BIOLOG identification system and molecular phylogenetic tree of 16S rDNA gene sequences,and the pathogenicity was investigated in a recursive infection experiment by intraperitoneal injection with bacteria suspension.Ten Chinese herbs were screened in attempt to test their inhibitory effects,from which ten double-and four triple-compound prescriptions were formulated and tested.Results show that the causative strain was Plesiomonas shigelloides,and the LD50 was 3.2×105 CFU/g.Study on antimicrobial susceptibility showed that the ten Chinese herbs had good antibacterial effect on the bacterium.Galla chinensis,Eugenia caryophllata,and Sanguisorba officinalis showed better antibacterial activity than others did.Seven double-and one triple-compound prescriptions showed synergistic inhibitory effect;prescriptions with Galla chinensis showed good control on pathogen P.shigelloides,and shall be recommended for protection and prevention,green and safe to the environment. |
| Key words: Anguilla bicolor pacifica Plesiomonas shigelloides pathogenic bacterium antimicrobial susceptibility test |
