| 摘要: |
| 为了探索鲣鱼（Katsuwonus pelamis）鱼头眼窝肉中的DHA（C22：6n3）和EPA（C20：5n3）的抗抑郁活性效果，采用现代食品分离技术分离提取鲣鱼眼窝肉中的DHA和EPA，并对其抗抑郁活性效果进行研究。研究结果发现，当DHA和EPA剂量分别为50，100和300mg/kg时，能够对强迫游泳实验和悬尾实验的小鼠产生抗抑郁作用，其中低剂量（50mg/kg）表现为最活跃。DHA和EPA还能够对引起头抽动综合征的5-羟色胺小鼠产生轻微的增强作用。通过高效液相色谱-电化学法同时测定小鼠大脑区域内主要的单胺类神经递质及其代谢产物。本实验发现，与用压力媒介物处理的小鼠相比，DHA和EPA能够显著增加主要神经递质5-羟色胺和去肾上腺素在小鼠海马体、下丘脑及皮质层中的浓度，并减少5-羟色胺的代谢。DHA和EPA能够在预测抗抑郁特性的动物模型中（强迫游泳实验，悬尾实验）明确其具有抗抑郁活性效果，具有潜在的生物医学应用前景和环保效应。 |
| 关键词: 鲣鱼 DHA EPA 抗抑郁活性 5-羟色胺 神经递质 |
| DOI：10.11693/hyhz20160600141 |
| 分类号: |
| 基金项目:浙江省科技计划项目，2016C32080号；舟山市科技计划项目，2014C41002号，2016C41003号。 |
附件 |
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| DHA AND EPA FROM ORBITAL FLESH OF TUNA KATSUWONUS PELAMIS: EXTRACTION AND ITS ANTIDEPRESSANT ACTIVITY |
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LI Hai-Bo1, LV Qian-Qian2, BAI Dong2, WANG Huan2, GUAN Li-Ping2, XIE Chao2
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1.Zhejiang International Maritime College, Zhoushan 316021, China;2.Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, College of Food and Medicine, Zhejiang Ocean University, Zhoushan 316022, China
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| Abstract: |
| To explore the use of leftover from skipjack tuna Katsuwonus pelamis food industry,the orbital flesh was utilized for DHA and EPA extraction and their effects of antidepressant activity were studied.The extracted DHA and EPA were oral administrated in dosage at 50,100,and 300mg/kg onto mice.Antidepressant effect was investigated via forced swimming test (FST) and tail suspension test (TST) on mice.Results show that the lower dose (50mg/kg) group showed the most active effect.In addition,the DHA and EPA produced a slight potentiation of 5-HT-induced head twitches and syndrome.In addition,major monoamine neurotransmitters and their metabolites in mouse brain regions were determined by HPLC-ECD (High performance liquid chromatography-electrochemical detection).It was found that the DHA and EPA could significantly increase the concentrations of the neurotransmitters 5-HT and norepinephrine in the hippocampus,hypothalamus,and cortex;and reduce the 5-HT metabolism.Therefore,the leftover can be reused to extract DHA and EPA for antidepressant treatment as revealed in mice test.The practice opened a green way to make use of industrial waste for the sakes of human health and better environment. |
| Key words: Katsuwonus pelamis DHA EPA antidepressant activity 5-hydroxytryptophan neuro-transmitters |
