首页 | 期刊简介 | 编委会 | 投稿指南 | 常用下载 | 联系我们 | 期刊订阅 | In English
引用本文:陈兴强,李荣华,王春琳,胡益鸣,程继生,王成,詹萍萍,母昌考,宋微微,龚小敏.紫贻贝(Mytilus edulis)与厚壳贻贝(M. coruscus)杂交的细胞学观察及子一代的早期生长比较.海洋与湖沼,2017,48(3):600-608.
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 2075次   下载 2177 本文二维码信息
码上扫一扫!
分享到: 微信 更多
紫贻贝(Mytilus edulis)与厚壳贻贝(M. coruscus)杂交的细胞学观察及子一代的早期生长比较
陈兴强1,2, 李荣华1,2, 王春琳1,2, 胡益鸣1, 程继生1, 王成1, 詹萍萍1,2, 母昌考1,2, 宋微微1,2, 龚小敏3
1.宁波大学 应用海洋生物技术教育部重点实验室 宁波 315211;2.浙江海洋高效健康养殖协同创新中心 宁波 315211;3.象山鹤浦镇人民政府海洋与渔业办公室 象山 315733
摘要:
为了研究紫贻贝与厚壳贻贝种间杂交的核相变化,并评估其杂交优势,本实验以两种贻贝亲本为材料建立了4个交配组合,分别为:紫贻贝自交组EE(Mytilus edulis♀×M. edulis♂)、厚壳贻贝自交组CC(Mytilus coruscus♀×M. coruscus ♂)、正交组EC(M. edulis♀×M. coruscus♂)和反交组CE(M. coruscus♀×M. edulis♂)。通过Hoechst 33258染色、荧光显微观察的方法,对4个交配组从受精、减数分裂到卵裂的核相变化过程进行了观察,并对孵化后幼虫到稚贝期的生长情况进行了记录。结果表明:正反交组的受精细胞学过程与自交组较为相似,但第二极体排出时间延长。对各实验组子一代早期的生长和存活研究表明:浮游阶段,杂交组幼虫生长优势不明显;附着变态后,正交组EC在壳长及壳高等生长指标的杂交优势表现明显,且正交组(EC)大于反交组(CE)。自交组的受精率、孵化率、成活率均高于杂交组。
关键词:  紫贻贝  厚壳贻贝  杂交  细胞学观察  生长比较
DOI:10.11693/hyhz20161200279
分类号:Q789;S968.3
基金项目:浙江省贻贝产业服务团队科技特派员项目,2013.09-2017.12;浙江省重中之重学科开放基金项目,xkzsc1529号;宁波市科技富民项目,2015C10006号;王宽诚基金资助。
附件
CYTOLOGICAL OBSERVATION ON NUCLEAR BEHAVIOR OF HYBRIDS BETWEEN MYTILUS EDULIS AND M. CORUSCUS AND COMPARISON IN EARLY-STAGE GROWTH
CHEN Xing-Qiang1,2, LI Rong-Hua1,2, WANG Chun-Lin1,2, HU Yi-Ming1, CHENG Ji-Sheng1, WANG Cheng1, ZHAN Ping-Ping1,2, MU Chang-Kao1,2, SONG Wei-Wei1,2, GONG Xiao-Min3
1.Key Laboratory of Applied Marine Biotechnology, Ministry of Education, School of Marine Science, Ningbo University, Ningbo 315211, China;2.Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China;3.Ocean and Fishery Office of People's Government of Hepu, Xiangshan 315733, China
Abstract:
To investigate nuclear behavior in meiosis and evaluate heterosis between Mytilus edulis and M. coruscus, four crosses were established:EE (Mytilus edulis♀×M. edulis♂), CC (Mytilus coruscus♀×M. coruscus♂), EC (M. edulis♀×M. coruscus♂) and CE (M. coruscus♀×M. edulis♂). The cytological changes of nuclear behavior during fertilization and early cleavage of the crosses were observed continually with fluorescent microscope with Hoechst 33258 stain, and the larval growths at early stage were compared. The results indicate that the cytological process of fertilization was nearly the same for interspecific and intraspecific groups, whereas the emission of the second polar body was prolonged in interspecific hybrid groups. The growth advantage of the hybrid groups were not very evident during planktonic stage; but EC group showed significant heterosis in the characteristics of shell length and shell height after metamorphosis. In addition, EC group had a greater growth advantage over CE group. The fertilization rate, hatching rate and survival rate of all the intraspecific groups were higher than those of the interspecific groups.
Key words:  Mytilus edulis  Mytilus coruscus  hybridization  cytological observation  growth comparison
版权所有 海洋与湖沼 Oceanologia et Limnlolgia Sinica Copyright©2008 All Rights Reserved
主管单位:中国科协技术协会 主办单位:中国海洋湖沼学会
地址:青岛市海军路88号  邮编:266400  电话:0532-82898753  E-mail:ols@qdio.ac.cn
技术支持:北京勤云科技发展有限公司