| 摘要: |
| 为丰富我国拟菱形藻属的物种多样性，并澄清其产毒特征，本文从我国东南海域分离和建立了拟菱形藻的单克隆培养株系，结合光学显微镜下的群体特征和透射电镜下的超微形态特征，还有基于核糖体转录间隔区的分子系统学数据，以及基于ITS2转录RNA的二级结构分析，鉴定到我国拟菱形藻属的2个新记录种：镰刀拟菱形藻（P.sabit Teng，Lim，Lim&Leaw）和中鼓拟菱形藻（P.inflatula（Hasle）Hasle）。镰刀拟菱形藻的典型形态特征是壳面呈现镰刀状，点条纹由两排孔纹组成，与并基拟菱形藻（P.decipiens）的系统学关系最为接近，ITS2-RNA有2个补偿碱基差异。中鼓拟菱形藻主要形态特征是壳面呈直线形，点条纹由单排孔纹组成，孔纹内部以上下两分区为主，与格氏拟菱形藻（P.granii）和亚弧线拟菱形藻（P.subcurvata）的亲缘关系接近，ITS2-RNA二级结构分别有4个补偿碱基差异。另外，利用高效液相色谱-质谱联用法对培养藻株的产毒特征进行了分析，未检测到多莫酸。本文为我国拟菱形藻属的物种多样性做了必要的补充，还可为拟菱形藻的毒理研究提供基础数据。 |
| 关键词: 镰刀拟菱形藻 中鼓拟菱形藻 物种多样性 形态 核糖体转录间隔区 ITS2-RNA二级结构 多莫酸 |
| DOI：10.11693/hyhz20161200288 |
| 分类号:Q949.2 |
| 基金项目:国家自然科学基金资助项目，31570205号，31370235号；广州市科技计划项目，201607010370号。 |
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| TWO NEW RECORDS OF DIATOM GENUS PSEUDO-NITZSCHIA AND THEIR DOMOIC ACID PRODUCTION IN SOUTHEASTERN CHINA COASTAL WATERS |
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HUANG Chun-Xiu, DONG Huan-Chang, LI Yang
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Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory of Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China
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| Abstract: |
| A set of monoclonal strains was isolated and established from the southeastern China coastal waters. These strains were identified carefully using morphology in light microscopy and transmission electron microscopy. Their molecular data from internal transcribed spacer region of ribosomal rRNA encoding gene were characterized, and compared with the secondary structure of ITS2 RNA transcripts. Two new recorded species were found for China, P. sabit Teng, Lim, Lim & Leaw and P. inflatula (Hasle) Hasle. Their morphology were compared with both morphology-similar and molecule-close taxa, and the unique molecular feature for the secondary structure of ITS2-RNA was analyzed. Meanwhile, the production of domoic acid was examined in liquid chromatography tandem mass spectrometry (LC-MS/MS) injection. However, it showed no detectable domoic acid. The new records enrich the diversity of the genus Pseudo-nitzschia in China, and may provide detail information of domoic acid production in Pseudo-nitzschia strains. |
| Key words: Pseudo-nitzschia sabit Pseudo-nitzschia inflatula diversity morphology internal transcribed spacer ITS2-RNA secondary structure domoic acid |
