| 摘要: |
| 本研究利用形态学方法和基于线粒体细胞色素氧化酶亚基I（COI）的遗传学方法分析了中国沿海青蚶（Barbatia virescens）6个地理群体的形态差异、遗传多样性、遗传结构及其种群历史动态。单因素方差分析（ANOVA）和Tukey检验表明，青蚶不同地理种群间表现出显著形态差异（P<0.05）。经PCR扩增测序获得长度为587bp的COI基因片段，112个个体共检测到18个多态性位点、17个单倍型，每个群体均有特有单倍型。青蚶群体的遗传多样性水平较低，总群体的平均单倍型多样度为0.5472，平均核苷酸多样度为0.000974。AMOVA分析表明，群体内个体间的遗传分化是青蚶群体遗传变异的主要原因，占87.40%。阳江群体与其他群体之间存在显著的低程度的遗传分化，而其他群体间的遗传分化不显著。单倍型网络关系图呈典型的星状拓扑结构，没有表现出显著的地理谱系结构。单倍型邻接树结果也没有明显分支，未呈现出地域性差异。中性检验和核苷酸不配对分析结果揭示青蚶种群历史上经历了群体扩事件，扩张时间约为26万年前。研究结果为青蚶资源的保护和可持续利用提供了基础资料。 |
| 关键词: 青蚶 细胞色素氧化酶亚基I 遗传多样性 遗传结构 种群历史动态 |
| DOI：10.11693/hyhz20170300058 |
| 分类号:S917.4 |
| 基金项目:国家自然科学基金项目，41276138号；教育部博士点基金，20130132110009号。 |
附件 |
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| POPULATION GENETIC STRUCTURE AND DEMOGRAPHIC HISTORY OF BARBATIA VIRESCENS ALONG CHINESE COAST BASED ON MITOCHONDRIAL COI SEQUENCES |
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WANG Ling, LI Qi, KONG Ling-Feng, YU Hong
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The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China
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| Abstract: |
| We investigated the genetic diversity, population genetic structure, and demographic history of Barbatia virescens along Chinese coasts. Six populations were selected including Shensi of Zhejiang, Nanji Island of Zhejiang, Xiapu of Fujian, Pingtan of Fujian, Yangjiang of Guangdong, and Beihai of Guangxi, among which morphological variations (P<0.05) were significant as revealed in ANOVA test. The mitochondrial cytochrome oxidase I (mtDNA COI) gene fragments of these populations were amplified and sequenced, from which 112 sequences of total length of 587bp nucleotide were obtained, including 18 polymorphic sites and 17 haplotypes. Overall, the mean genetic diversity was 0.5472, and mean nucleotide diversity 0.000974, suggesting that genetic diversity of B. virescens was relatively low. Most of the genetic variation was intra-population variation (87.40%) as indicated in AMOVA analysis. The population pairwise Fst values among populations were low, and no significant genetic differentiation was observed, except for Yangjiang population. In addition, no clear geographical differentiation was revealed in the haplotype network and on the NJ tree. The historical expansion time started about 2.6×105 years ago determined by Tajima's D and Fu's Fs tests, and mismatch distributions. These findings would offer insights to the fine-tune conservation and fishery management measures in the future. |
| Key words: Barbatia virescens mitochondrial COI genetic diversity population structure demography history |
