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翘嘴鳜(Siniperca chuatsi)EST-SSR标记与生长性状相关性及4个选育群体遗传结构研究
袁文成1, 叶金明2, 黄鹤忠1,3, 肖攀1, 路瑶1, 李泽1, 朱传坤3
1.苏州大学基础医学与生物科学学院 苏州大学水产研究所 苏州 215123;2.扬州市水产生产技术指导站 扬州 225007;3.江苏省区域现代农业与环境保护协同创新中心 淮安 223300
摘要:
采用翘嘴鳜(Siniperca chuatsi)转录组高通量测序并筛选得到的13对多态性EST-SSR引物,对60尾翘嘴鳜的体长、体高、体厚及体重4种生长性状进行标记-性状相关性研究,并对4个翘嘴鳜选育群体各30尾个体进行遗传结构分析。结果显示,与其生长性状显著相关的微卫星分子标记有5个,包括:分别与体厚和体长极显著相关(P<0.01)和显著相关(P<0.05)的标记C10127,AA型为体长、体厚的优势基因型,而AC型为劣势基因型;与体重显著相关(P<0.05)的标记C19016,AA型为体重的优势基因型,AB型为体长的劣势基因型;与体厚显著相关(P<0.05)的标记C14847,BB型为体厚的优势基因型;分别与体长、体高和体重、体厚极显著相关(P<0.01)和显著相关(P<0.05)的标记C12350,BC型为体长、体厚、体高和体重性状的优势基因型;分别与体厚、体重和体高极显著相关(P<0.01)和显著相关(P<0.05)的C18691标记,BB型为体厚、体重和体高性状的优势基因型。4个翘嘴鳜群体遗传多样性高低依次为太湖选育群体>长江口选育群体>洪泽湖选育群体>鄱阳湖选育群体,其平均的等位基因数、有效等位基因数、观测杂合度、期望杂合度和多态信息含量分别为2.2115、1.9405、0.55513、0.5925和0.3775,属于中度多态水平。
关键词:  翘嘴鳜  EST-SSR标记  生长性状  遗传结构  相关性分析
DOI:10.11693/hyhz20171000257
分类号:Q953;Q789
基金项目:江苏省水产三新工程项目,D2015-15号;江苏省科技计划现代农业重点项目,BE2017311号;江苏省区域现代农业与环境保护协同创新项目,48SKY00号;江苏省渔业科技项目,Y2017-38号;苏州市科技计划农业应用基础研究项目,SNG201608号。
STUDY ON CORRELATION OF EST-SSR MARKERS WITH GROWTH TRAITS AND GENETIC STRUCTURE OF 4 BREEDING POPULATIONS IN MANDARIN FISH SINIPERCA CHUATSI
YUAN Wen-Cheng1, YE Jin-Ming2, HUANG He-Zhong1,3, XIAO Pan1, LU Yao1, LI Ze1, ZHU Chuan-Kun3
1.School of Medicine and Life Sciences, Medical College of Soochow University, Fisheries Research Institute of Soochow University, Suzhou 215123, China;2.Aquatic Products Production Technical Guidance Station of Yangzhou City, Yangzhou 225007, China;3.Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huai'an 223300, China
Abstract:
The linkage between growth traits (body length, body height, body weight, and thickness) of 60 individuals and EST-SSR marker trait and genetic structure of 4 breeding groups (each having 30 individuals of mandarin fish Siniperca chuatsi) was analyzed by 13 polymorphic EST-SSR microsatellites obtained by transcriptome high-throughput sequencing and screening. The linkage analysis showed that:the locus C10127 had an extremely significant impact on body thickness (P<0.01) and a significant impact on body length (P<0.05). AA at C10127 was a favorable genotype for body length and thickness traits while AC was a disadvantage genotype. Locus C19016 showed a significant impact on body weight (P<0.05), in which AA was the dominant genotype of body weight while the AB was the negative genotype of body length. Locus C14847 showed a significant impact on thickness (P<0.05), and BB was advantage genotype of thickness. Locus C12350 presented an extremely significant impact on body length and body height (P<0.01) and a significant impact on body weight and thickness (P<0.05), in which BC was dominant genotype of the 4 growth traits. Locus C18691 showed an extremely significant impact on body weight and thickness (P<0.01), and a significant impact on body height (P<0.05), and BB was a dominant genotype of thickness, body weight, and body height. The genetic diversity analysis determined that the value of mean valid alleles was 1.9405, the number of mean expected heterozygosity was 0.5925 and the mean polymorphism information content was 0.3775, indicating intermediate level of polymorphism.
Key words:  Siniperca chuatsi  EST-SSR marker  growth trait  genetic structure  correlation analysis
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