| 摘要: |
| 本文采用Biolog-ECO微平板技术，分析了不同水力停留时间（hydraulic retention time，HRT）5、6、7h和进水硝酸盐（NO3--N）3浓度50、100、150mg/L时，好氧反硝化反应器微生物群落结构和代谢功能特征。研究结果表明：在同一进水硝酸盐浓度下，水力停留时间越长，微生物代谢活性越强（P<0.05）；在同一水力停留时间下，不同进水NO3-浓度下微生物平均吸光值（用平均颜色变化率AWCD指示）的大小顺序为50 > 150 > 100mg/L （P<0.05），说明进水NO3--N浓度对微生物代谢活性有一定影响。反应器内微生物对不同碳源的代谢利用由强到弱的顺序是：多聚物 > 氨基类 > 碳水化合物 > 羧酸类 > 胺类 > 酚酸类。不同处理组的Shannon-Wiener指数、Simpson指数、Pielou指数、McIntosh指数相互之间差异显著（P<0.05），其中HRT为7h、NO3-为150mg/L以上处理组微生物多样性指数最高。本实验采用Biolog-ECO板来分析在好氧反硝化反应器中微生物的群落代谢特征，研究结果可为通过碳源调节生物滤池水处理效果提供科学依据，以此提高水处理效率。 |
| 关键词: 好氧反硝化 微生物反应器 水力停留时间 硝酸盐浓度 Biolog-ECO板 碳源代谢 |
| DOI：10.11693/hyhz20170600163 |
| 分类号: |
| 基金项目:国家自然科学基金项目，31502212号。 |
附件 |
|
| CARBON METABOLISM OF MICROBIAL COMMUNITIES IN AEROBIC DENITRIFICATION REACTOR |
|
JIANG Yu-Li, HUANG Zhi-Tao, SONG Xie-Fa, CHEN Zhao
|
|
College of Fisheries, Ocean University of China, Qingdao 266003, China
|
| Abstract: |
| To understand the microbial community structure and carbon metabolism characteristic of aerobic denitrification reactor, the Biolog ECO technology was used to study the effects of hydraulic retention time (HRT) (5, 6, and 7h) and nitrate concentration (50, 100, and 150mg/L). The results show that the microbial metabolic activity was positively related to HRT within the range of the experiment. The order of impact of different nitrate concentration on AWCD (average well color development) was 50mg/L > 150mg/L > 100mg/L, showing that nitrate concentration affected the microbial metabolic activity. The preference order of various carbon sources for the microorganism in the denitrification reactor was polymers > acids amino > carbohydrates > carboxylic acids > amines > phenolic acids. The Shannon-Wiener index, Simpson dominance index, Pielou evenness index, and McIntosh index of various treatments were significantly different (P<0.05) between the treatments in pairs. Among them, the treatment whose HRT was 7h and NO-N was 150mg/L had the highest biodiversity. This experiment used the Biolog-ECO board to analyze the metabolism characteristics of the microbial community in the aerobic denitrifying reactor, and the results provided a new way to promote water treatment effect by adjusting carbon source. |
| Key words: aerobic denitrification microbial reactor hydraulic retention time (HRT) nitrate concentration Biolog-ECO board metabolism of carbon source |
