| 摘要: |
| 为了探明五氯苯酚暴露后花鲈（Lateolabrax japonicus）肝脏的损伤变化，本文利用Illumina HiSeqTM2000测序技术对不同浓度五氯苯酚（0，0.1，1.0，10.0，100μg/L）暴露后花鲈肝脏组织进行了转录组测序。经Trinity软件拼接，得到clean reads共247914284条，获得53716条基因。对照不同浓度暴露组和对照组的转录组测序结果，获得共有的差异性表达基因135个，特异性的差异基因数分别有127-819个不等，差异蛋白表达数量随着受试浓度的升高而上升。经BLAST搜索，其中有21459、26464、18896、12403、17262和19159条分别注释到Swiss-prot、Nr、Pfam、KEGG、KOG和GO数据库。KEGG代谢通路分析结果显示获得的差异基因分别映射到6大类141条通路，这些差异表达基因在与环境信息进程相关的信号传导和与组织系统相关的免疫系统的通路中富集最多。KOG数据库预测和分类表明16571条基因归属于25大类，参与一般功能预测和信号传导机制的数量最多，参与翻译后修饰、蛋白质转换与分子伴侣路径的也占较大比例。总体来看，在受到五氯苯酚类物质胁迫后，花鲈体内的信号传导系统首先发生响应，导致机体出现紊乱。污染物暴露后，花鲈机体的应对机制尚待深入分析。 |
| 关键词: 花鲈 转录组 五氯苯酚 差异性表达基因 |
| DOI：10.11693/hyhz20170500133 |
| 分类号:Q955;S949;X55 |
| 基金项目:公益性行业（农业）科研专项，201303047号；宁波市科技局重点项目，2013C11027号。 |
附件 |
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| Transcriptomic profilES of lateolabrax japonicus liver INJURED by Pentachlorophenol stress |
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ZHENG Dan1,2, JIAO Hai-Feng2, YAN Xiao-Jun1, ZHANG Le3, RUAN Song-Lin3
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1.School of Marine Science, Ningbo University, Ningbo 315211, China;2.Ningbo Academy of Oceanology and Fishery, Ningbo 315103, China;3.Hangzhou Academy of Agricultural Sciences, Hangzhou 310021, China
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| Abstract: |
| To analyze the toxic mechanism of Lateolabrax japonicus liver injury after exposure to different concentrations of pentachlorophenol (0, 0.1, 1.0, 10.0, and 100μg/L), we conducted the transcriptome sequencing and analysis by Illumina HiSeqTM2000. A total of 247914284 clean reads and 53716 genes were determined by software Trinity. By comparing the four stress groups with the control, the number of common differentially expressed genes was 135, and the number of specific differentially expressed genes was from 127 to 819. These unigenes were partially matched with known proteins in the National Center for Biotechnology Information (NCBI) database, as determined by BLAST search, with 21459, 26464, 18896, 12403, 17262, and 19159 unigenes assigned to Swiss-Prot, Nr database, Pfam database, KEGG, KOG and Gene Ontology (GO), respectively. The KEGG pathway analysis indicated that differentially expressed genes were enriched in 141 pathways of six categories. These differentially expressed genes were enriched in the signaling pathways that associated with environmental information process and immune system, and as well as in the signal endocrine system, cell communication, transport and catabolic processes. The result of KOG database prediction and classification indicates that 16571 genes are enriched in the 25 categories. About these genes, the function of general functional predictions and signal transduction mechanisms accounted for the largest proportion. The pathways involved in post-translational modification, protein conversion, and molecular chaperone pathways also accounted for a large proportion. Overall, the signal transduction system of L. japonicus was first responded after pentachlorophenol stress, resulting in the body disorder. |
| Key words: Lateolabrax japonicus transcriptome pentachlorophenol differentially expressed genes |
