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牛磺酸和甲硫氨酸亚砜亚胺对高血氨症大弹涂鱼氧化应激和炎症反应相关基因影响的比较研究
邢晓丹1, 张木子2, 黎明2, 袁莉霞2, 王日昕2, 石戈1
1.浙江海洋大学海洋科学与技术学院 舟山 316022;2.宁波大学海洋学院 宁波 315211
摘要:
为了查明氨中毒对大弹涂鱼的影响,以及牛磺酸和甲硫氨酸亚砜亚胺对鱼类高血氨症的缓释作用,本研究设置了4个处理组:组1腹腔注射氯化钠,组2腹腔注射醋酸铵,组3腹腔注射牛磺酸和醋酸铵,组4腹腔注射甲硫氨酸亚砜亚胺和醋酸铵。实验持续96h。结果发现,组2实验鱼肝脏中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽巯基转移酶基因的mRNA表达量显著最高,其次是组1,而组3和组4显著最低。同样,组2实验鱼肝脏中白细胞介素6(IL6)和IL 8显着高于其他组。肿瘤坏死因子基因的mRNA表达量,组1显著高于其他组,而IL 1基因的mRNA表达量,组4显著最高。结果表明:氨氮毒性能够影响鱼体内抗氧化酶系统及免疫应答,导致氧化损伤和炎症反应;外源性牛磺酸能够缓解鱼类高血氨症带来的负面影响;甲硫氨酸亚砜亚胺尽管能够缓解自由基造成的氧化损伤,但对炎症反应并未发挥有效的作用。
关键词:  氨氮  牛磺酸  甲硫氨酸亚砜亚胺  氧化应激  炎症  大弹涂鱼
DOI:10.11693/hyhz20171100285
分类号:Q789;S941
基金项目:国家自然科学基金项目,31472279号,31502176号;宁波市自然科学基金项目,2016A610083号。
COMPARISON EFFECTS OF TAURINE AND METHIONINE SULFOXIMINE ON GENES INVOLVED IN OXIDATIVE STRESS AND INFLAMMATION OF HYPERAMMONEMIA MUDSKIPPER
Xing Xiao-Dan1, Zhang Mu-Zi2, Li Ming2, Yuan Li-Xia2, Wang Ri-Xin2, Shi Ge1
1.School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China;2.School of Marine Science, Ningbo University, Ningbo 315211, China
Abstract:
Four experimental groups were set to test the response of mudskipper to ammonia toxicity and the mitigation effects of taurine and methionine sulfoximine (MSO) on the toxicity. Group 1 was injected with NaCl, Group 2 with CH3COONH4, Group 3 with CH3COONH4 and taurine, and Group 4 CH3COONH4 and MSO. The experiment lasted for 96h. Results show that fish in Group 2 had the highest mRNA expression levels of superoxide dismutase, catalase, glutathione peroxidase, and glutathione s-transferase, followed by Group 1, and the lowest in Groups 3 and 4. Similarly, the expression levels of interleukin 6 (IL 6) and IL 8 of fish in Group 2 were significantly higher than those of other groups. But fish in control group had the highest mRNA expression level of tumor necrosis factor. In comparison, fish in Group 4 had the highest expression level of IL 1. Therefore, ammonia poisoning in fish would lead to oxidative damage and inflammation The exogenous taurine could mitigate the adverse effect of fish hyperammonemia. Although MSO could ease the oxidative damage, it did not always regulate the inflammation effectively.
Key words:  ammonia  taurine  methionine sulfoximine (MSO)  oxidative stress  inflammation  mudskipper Boleophthalmus pectinirostris
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